tetano
Editor, Senior Moderator
J Clin Microbiol
. 2020 Jul 7;JCM.01438-20.
doi: 10.1128/JCM.01438-20. Online ahead of print.
Clinical Evaluation of Self-Collected Saliva by RT-qPCR, Direct RT-qPCR, RT-LAMP, and a Rapid Antigen Test to Diagnose COVID-19
Mayu Nagura-Ikeda[SUP] 1 [/SUP], Kazuo Imai[SUP] 2 3 [/SUP], Sakiko Tabata[SUP] 1 [/SUP], Kazuyasu Miyoshi[SUP] 1 [/SUP], Nami Murahara[SUP] 1 [/SUP], Tsukasa Mizuno[SUP] 1 [/SUP], Midori Horiuchi[SUP] 1 [/SUP], Kento Kato[SUP] 1 [/SUP], Yoshitaka Imoto[SUP] 1 [/SUP], Maki Iwata[SUP] 1 [/SUP], Satoshi Mimura[SUP] 1 [/SUP], Toshimitsu Ito[SUP] 1 [/SUP], Kaku Tamura[SUP] 1 [/SUP], Yasuyuki Kato[SUP] 4 [/SUP]
Affiliations
Abstract
Background: The clinical performance of six molecular diagnostic tests and a rapid antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were clinically evaluated for the diagnosis of coronavirus disease 2019 (COVID-19) in self-collected saliva.Methods: Saliva samples from 103 patients with laboratory-confirmed COVID-19 (15 asymptomatic and 88 symptomatic) were collected on the day of hospital admission. SARS-CoV-2 RNA in saliva was detected using a quantitative reverse-transcription polymerase chain reaction (RT-qPCR) laboratory-developed test (LDT), a cobas SARS-CoV-2 high-throughput system, three direct RT-qPCR kits, and reverse-transcription loop mediated isothermal amplification (RT-LAMP). The viral antigen was detected by a rapid antigen immunochromatographic assay.Results: Of the 103 samples, viral RNA was detected in 50.5-81.6% of the specimens by molecular diagnostic tests and an antigen was detected in 11.7% of the specimens by the rapid antigen test. Viral RNA was detected at a significantly higher percentage (65.6-93.4%) in specimens collected within 9 d of symptom onset compared to that of specimens collected after at least 10 d of symptom onset (22.2-66.7%) and that of asymptomatic patients (40.0-66.7%).Conclusions: Self-collected saliva is an alternative specimen option for diagnosing COVID-19. LDT RT-qPCR, cobas SARS-CoV-2 high-throughput system, direct RT-qPCR except for one commercial kit, and RT-LAMP showed sufficient sensitivity in clinical use to be selectively used according to clinical settings and facilities. The rapid antigen test alone is not recommended for initial COVID-19 diagnosis because of its low sensitivity.
. 2020 Jul 7;JCM.01438-20.
doi: 10.1128/JCM.01438-20. Online ahead of print.
Clinical Evaluation of Self-Collected Saliva by RT-qPCR, Direct RT-qPCR, RT-LAMP, and a Rapid Antigen Test to Diagnose COVID-19
Mayu Nagura-Ikeda[SUP] 1 [/SUP], Kazuo Imai[SUP] 2 3 [/SUP], Sakiko Tabata[SUP] 1 [/SUP], Kazuyasu Miyoshi[SUP] 1 [/SUP], Nami Murahara[SUP] 1 [/SUP], Tsukasa Mizuno[SUP] 1 [/SUP], Midori Horiuchi[SUP] 1 [/SUP], Kento Kato[SUP] 1 [/SUP], Yoshitaka Imoto[SUP] 1 [/SUP], Maki Iwata[SUP] 1 [/SUP], Satoshi Mimura[SUP] 1 [/SUP], Toshimitsu Ito[SUP] 1 [/SUP], Kaku Tamura[SUP] 1 [/SUP], Yasuyuki Kato[SUP] 4 [/SUP]
Affiliations
- PMID: 32636214
- DOI: 10.1128/JCM.01438-20
Abstract
Background: The clinical performance of six molecular diagnostic tests and a rapid antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were clinically evaluated for the diagnosis of coronavirus disease 2019 (COVID-19) in self-collected saliva.Methods: Saliva samples from 103 patients with laboratory-confirmed COVID-19 (15 asymptomatic and 88 symptomatic) were collected on the day of hospital admission. SARS-CoV-2 RNA in saliva was detected using a quantitative reverse-transcription polymerase chain reaction (RT-qPCR) laboratory-developed test (LDT), a cobas SARS-CoV-2 high-throughput system, three direct RT-qPCR kits, and reverse-transcription loop mediated isothermal amplification (RT-LAMP). The viral antigen was detected by a rapid antigen immunochromatographic assay.Results: Of the 103 samples, viral RNA was detected in 50.5-81.6% of the specimens by molecular diagnostic tests and an antigen was detected in 11.7% of the specimens by the rapid antigen test. Viral RNA was detected at a significantly higher percentage (65.6-93.4%) in specimens collected within 9 d of symptom onset compared to that of specimens collected after at least 10 d of symptom onset (22.2-66.7%) and that of asymptomatic patients (40.0-66.7%).Conclusions: Self-collected saliva is an alternative specimen option for diagnosing COVID-19. LDT RT-qPCR, cobas SARS-CoV-2 high-throughput system, direct RT-qPCR except for one commercial kit, and RT-LAMP showed sufficient sensitivity in clinical use to be selectively used according to clinical settings and facilities. The rapid antigen test alone is not recommended for initial COVID-19 diagnosis because of its low sensitivity.