Giuseppe
Emeritus
J Clin Microbiol. 2009 Jan 7. [Epub ahead of print]
A Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for the Confirmation of Respiratory Syncytial Virus and Influenza Viruses Detected by Antigen Immunoassay Testing.
Liao RS, Tomalty LL, Majury A, Zoutman DE. - Division of Medical Microbiology and Infection Control, Department of Pathology and Molecular Medicine, and Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassay testing with the BD Directigen A+B and Binax NOW RSV assays over 2 successive respiratory virus seasons.
Ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 119 were positive for RSV and 59 were positive for influenza virus.
The median age of the patients with specimens positive for RSV and influenza were 6.3 mo and 42.4 yr, respectively.
The specificity for all of the methods tested was >/=99% and the individual sensitivities of the NOW RSV, RSV culture, Directigen A+B, influenza culture, and the Proflu-1 PCR for influenza/RSV were: 82% (95% confidence intervals [CI]: 73-88), 57% (95% CI: 44-69), 59% (95% CI: 44-72), 54% (95% CI: 38-69), and 98% (95% CI: 93-100)/95% (95% CI: 85-99).
In a clinical setting where viral isolation is performed to confirm rapid antigen immunoassay results of these common respiratory viruses, one-step real-time reverse transcriptase PCR testing can be a more sensitive and timely confirmatory method.
PMID: 19129410 [PubMed - as supplied by publisher]
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A Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for the Confirmation of Respiratory Syncytial Virus and Influenza Viruses Detected by Antigen Immunoassay Testing.
Liao RS, Tomalty LL, Majury A, Zoutman DE. - Division of Medical Microbiology and Infection Control, Department of Pathology and Molecular Medicine, and Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassay testing with the BD Directigen A+B and Binax NOW RSV assays over 2 successive respiratory virus seasons.
Ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 119 were positive for RSV and 59 were positive for influenza virus.
The median age of the patients with specimens positive for RSV and influenza were 6.3 mo and 42.4 yr, respectively.
The specificity for all of the methods tested was >/=99% and the individual sensitivities of the NOW RSV, RSV culture, Directigen A+B, influenza culture, and the Proflu-1 PCR for influenza/RSV were: 82% (95% confidence intervals [CI]: 73-88), 57% (95% CI: 44-69), 59% (95% CI: 44-72), 54% (95% CI: 38-69), and 98% (95% CI: 93-100)/95% (95% CI: 85-99).
In a clinical setting where viral isolation is performed to confirm rapid antigen immunoassay results of these common respiratory viruses, one-step real-time reverse transcriptase PCR testing can be a more sensitive and timely confirmatory method.
PMID: 19129410 [PubMed - as supplied by publisher]
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