tetano
Editor, Senior Moderator
J Clin Med
. 2020 Nov 5;9(11):E3569.
doi: 10.3390/jcm9113569.
Seroprevalence of SARS-CoV-2 IgG Antibodies in Corsica (France), April and June 2020
Lisandru Capai[SUP] 1 [/SUP], Nazli Ayhan[SUP] 1 2 [/SUP], Shirley Masse[SUP] 1 [/SUP], Jean Canarelli[SUP] 3 [/SUP], St?phane Priet[SUP] 2 [/SUP], Marie-H?l?ne Simeoni[SUP] 4 [/SUP], Remi Charrel[SUP] 2 [/SUP], Xavier de Lamballerie[SUP] 2 [/SUP], Alessandra Falchi[SUP] 1 [/SUP]
Affiliations
Abstract
Our aim was to assess the seroprevalence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection after the lockdown in a sample of the Corsican population. Between 16 April and 15 June 2020, 2312 residual sera were collected from patients with a blood analysis conducted in one of the participating laboratories. Residual sera obtained from persons of all ages were tested for the presence of anti-SARS-CoV-2 Immunoglobulin G (IgG) using the EUROIMMUN enzyme immunoassay kit for semiquantitative detection of IgG antibodies against the S1 domain of viral spike protein (ELISA-S). Borderline and positive samples in ELISA-S were also tested with an in-house virus neutralization test (VNT). Prevalence values were adjusted for sex and age. A total of 1973 residual sera samples were included in the study. The overall seroprevalence based on ELISA-S was 5.27% (95% confidence interval (CI), 4.33-6.35) and 5.46% (4.51-6.57) after adjustment. Sex was not associated with IgG detection. However, significant differences were observed between age groups (p-value = 1 E-5). The highest values were observed among 10-19, 30-39, and 40-49 year-old age groups, ranging around 8-10%. The prevalence of neutralizing antibody titers ≥40 was 3% (2.28-3.84). In conclusion, the present study showed a low seroprevalence for COVID-19 in Corsica, a finding that is in accordance with values reported for other French regions in which the impact of the pandemic was low.
Keywords: ELISA; France; SARS-CoV-2; antibodies; seroneutralization; seroprevalence.
. 2020 Nov 5;9(11):E3569.
doi: 10.3390/jcm9113569.
Seroprevalence of SARS-CoV-2 IgG Antibodies in Corsica (France), April and June 2020
Lisandru Capai[SUP] 1 [/SUP], Nazli Ayhan[SUP] 1 2 [/SUP], Shirley Masse[SUP] 1 [/SUP], Jean Canarelli[SUP] 3 [/SUP], St?phane Priet[SUP] 2 [/SUP], Marie-H?l?ne Simeoni[SUP] 4 [/SUP], Remi Charrel[SUP] 2 [/SUP], Xavier de Lamballerie[SUP] 2 [/SUP], Alessandra Falchi[SUP] 1 [/SUP]
Affiliations
- PMID: 33167563
- DOI: 10.3390/jcm9113569
Abstract
Our aim was to assess the seroprevalence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection after the lockdown in a sample of the Corsican population. Between 16 April and 15 June 2020, 2312 residual sera were collected from patients with a blood analysis conducted in one of the participating laboratories. Residual sera obtained from persons of all ages were tested for the presence of anti-SARS-CoV-2 Immunoglobulin G (IgG) using the EUROIMMUN enzyme immunoassay kit for semiquantitative detection of IgG antibodies against the S1 domain of viral spike protein (ELISA-S). Borderline and positive samples in ELISA-S were also tested with an in-house virus neutralization test (VNT). Prevalence values were adjusted for sex and age. A total of 1973 residual sera samples were included in the study. The overall seroprevalence based on ELISA-S was 5.27% (95% confidence interval (CI), 4.33-6.35) and 5.46% (4.51-6.57) after adjustment. Sex was not associated with IgG detection. However, significant differences were observed between age groups (p-value = 1 E-5). The highest values were observed among 10-19, 30-39, and 40-49 year-old age groups, ranging around 8-10%. The prevalence of neutralizing antibody titers ≥40 was 3% (2.28-3.84). In conclusion, the present study showed a low seroprevalence for COVID-19 in Corsica, a finding that is in accordance with values reported for other French regions in which the impact of the pandemic was low.
Keywords: ELISA; France; SARS-CoV-2; antibodies; seroneutralization; seroprevalence.