tetano
Editor, Senior Moderator
J Clin Lab Anal
. 2022 Jun 9;e24545.
doi: 10.1002/jcla.24545. Online ahead of print.
Heterogeneity assessment of vaccine-induced effects using point-of-care surrogate neutralization test for severe acute respiratory syndrome coronavirus 2
Yoshiyuki Watanabe[SUP] 1 2 3 4 5 [/SUP], Ikuro Matsuba[SUP] 1 [/SUP], Karin Watanabe[SUP] 2 [/SUP], Tomoyuki Kunishima[SUP] 1 [/SUP], Yukako Takechi[SUP] 1 [/SUP], Tetsuo Takuma[SUP] 1 [/SUP], Yasushi Araki[SUP] 1 [/SUP], Nobuo Hirotsu[SUP] 1 [/SUP], Hiroyuki Sakai[SUP] 1 [/SUP], Ritsuko Oikawa[SUP] 3 [/SUP], Hiroki Danno[SUP] 6 [/SUP], Masakazu Fukuda[SUP] 6 [/SUP], Seiji Futagami[SUP] 4 [/SUP], Kota Wada[SUP] 5 [/SUP], Hiroyuki Yamamoto[SUP] 7 [/SUP], Fumio Itoh[SUP] 3 [/SUP], Ichiro Oda[SUP] 2 [/SUP], Yutaka Hatori[SUP] 1 [/SUP], Hisakazu Degawa[SUP] 1 [/SUP]
Affiliations
Abstract
Introduction: Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic even after vaccination. We aimed to identify immunological heterogeneity over time in vaccinated healthcare workers using neutralization antibodies and neutralizing activity tests.
Methods: Serum samples were collected from 214 healthcare workers before vaccination (pre) and on days 22, 90, and 180 after receiving the first dose of BNT162b2 vaccine (day 0). Neutralization antibody (NAb, SARS-CoV-2 S-RBD IgM/IgG) titers and two kinds of surrogate virus neutralization tests (sVNTs) were analyzed (UMIN000043851).
Results: The NAb (SARS-CoV-2 S-RBD IgG) titer peaked on day 90 after vaccination (30,808.0 μg/ml ± 35,211; p < 0.0001) and declined on day 180 (11,678.0 μg/ml ± 33,770.0; p < 0.0001). The neutralizing activity also peaked on day 90 and declined with larger individual differences than those of IgG titer on day 180 (88.9% ± 15.0%, 64.8% ± 23.7%, p < 0.0001). We also found that the results of POCT-sVNT (immunochromatography) were highly correlated with those of conventional sVNT (ELISA).
Conclusions: Neutralizing activity is the gold standard for vaccine efficacy evaluation. Our results using conventional sVNT showed large individual differences in neutralizing activity reduction on day 180 (64.8% ± 23.7%), suggesting an association with the difference in vaccine efficacy. POCT-sVNT is rapid and user-friendly; it might be used for triage in homes, isolation facilities, and event venues without restrictions on the medical testing environment.
Keywords: COVID-19; POCT; SARS-CoV-2; neutralizing activity; sVNT.
. 2022 Jun 9;e24545.
doi: 10.1002/jcla.24545. Online ahead of print.
Heterogeneity assessment of vaccine-induced effects using point-of-care surrogate neutralization test for severe acute respiratory syndrome coronavirus 2
Yoshiyuki Watanabe[SUP] 1 2 3 4 5 [/SUP], Ikuro Matsuba[SUP] 1 [/SUP], Karin Watanabe[SUP] 2 [/SUP], Tomoyuki Kunishima[SUP] 1 [/SUP], Yukako Takechi[SUP] 1 [/SUP], Tetsuo Takuma[SUP] 1 [/SUP], Yasushi Araki[SUP] 1 [/SUP], Nobuo Hirotsu[SUP] 1 [/SUP], Hiroyuki Sakai[SUP] 1 [/SUP], Ritsuko Oikawa[SUP] 3 [/SUP], Hiroki Danno[SUP] 6 [/SUP], Masakazu Fukuda[SUP] 6 [/SUP], Seiji Futagami[SUP] 4 [/SUP], Kota Wada[SUP] 5 [/SUP], Hiroyuki Yamamoto[SUP] 7 [/SUP], Fumio Itoh[SUP] 3 [/SUP], Ichiro Oda[SUP] 2 [/SUP], Yutaka Hatori[SUP] 1 [/SUP], Hisakazu Degawa[SUP] 1 [/SUP]
Affiliations
- PMID: 35678628
- DOI: 10.1002/jcla.24545
Abstract
Introduction: Coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic even after vaccination. We aimed to identify immunological heterogeneity over time in vaccinated healthcare workers using neutralization antibodies and neutralizing activity tests.
Methods: Serum samples were collected from 214 healthcare workers before vaccination (pre) and on days 22, 90, and 180 after receiving the first dose of BNT162b2 vaccine (day 0). Neutralization antibody (NAb, SARS-CoV-2 S-RBD IgM/IgG) titers and two kinds of surrogate virus neutralization tests (sVNTs) were analyzed (UMIN000043851).
Results: The NAb (SARS-CoV-2 S-RBD IgG) titer peaked on day 90 after vaccination (30,808.0 μg/ml ± 35,211; p < 0.0001) and declined on day 180 (11,678.0 μg/ml ± 33,770.0; p < 0.0001). The neutralizing activity also peaked on day 90 and declined with larger individual differences than those of IgG titer on day 180 (88.9% ± 15.0%, 64.8% ± 23.7%, p < 0.0001). We also found that the results of POCT-sVNT (immunochromatography) were highly correlated with those of conventional sVNT (ELISA).
Conclusions: Neutralizing activity is the gold standard for vaccine efficacy evaluation. Our results using conventional sVNT showed large individual differences in neutralizing activity reduction on day 180 (64.8% ± 23.7%), suggesting an association with the difference in vaccine efficacy. POCT-sVNT is rapid and user-friendly; it might be used for triage in homes, isolation facilities, and event venues without restrictions on the medical testing environment.
Keywords: COVID-19; POCT; SARS-CoV-2; neutralizing activity; sVNT.