tetano
Editor, Senior Moderator
J Clin Immunol
. 2022 Jan 9.
doi: 10.1007/s10875-021-01190-5. Online ahead of print.
Seasonal Betacoronavirus Antibodies' Expansion Post-BNT161b2 Vaccination Associates with Reduced SARS-CoV-2 VoC Neutralization
Stefania Dispinseri[SUP] 1 [/SUP], Ilaria Marzinotto[SUP] 2 [/SUP], Cristina Brigatti[SUP] 2 [/SUP], Maria Franca Pirillo[SUP] 3 [/SUP], Monica Tolazzi[SUP] 1 [/SUP], Elena Bazzigaluppi[SUP] 2 [/SUP], Andrea Canitano[SUP] 3 [/SUP], Martina Borghi[SUP] 4 [/SUP], Alessandra Gallinaro[SUP] 3 [/SUP], Roberta Caccia[SUP] 5 [/SUP], Riccardo Vercesi[SUP] 5 [/SUP], Paul F McKay[SUP] 6 [/SUP], Fabio Ciceri[SUP] 7 8 [/SUP], Lorenzo Piemonti[SUP] 2 7 [/SUP], Donatella Negri[SUP] 4 [/SUP], Paola Cinque[SUP] 5 [/SUP], Andrea Cara[SUP] 3 [/SUP], Gabriella Scarlatti[SUP] 9 [/SUP], Vito Lampasona[SUP] 10 [/SUP]
Affiliations
Abstract
SARS-CoV-2 vaccination is known to induce antibodies that recognize also variants of concerns (VoCs) of the virus. However, epidemiological and laboratory evidences indicate that these antibodies have a reduced neutralization ability against VoCs. We studied binding and neutralizing antibodies against the Spike protein domains and subunits of the Wuhan-Hu-1 virus and its alpha, beta, delta VoCs and of seasonal betacoronaviruses (HKU1 and OC43) in a cohort of 31 health care workers prospectively followed post-vaccination with BNT162b2-Comirnaty. The study of sequential samples collected up to 64 days post-vaccination showed that serological assays measuring IgG against Wuhan-Hu-1 antigens were a poor proxy for VoC neutralization. In addition, in subjects who had asymptomatic or mild COVID-19 prior to vaccination, the loss of nAbs following disease could be rapid and accompanied by post-vaccination antibody levels similar to those of naïve vaccinees. Interestingly, in health care workers naïve for SARS-CoV-2 infection, vaccination induced a rapid and transient reactivation of pre-existing seasonal coronaviruses IgG responses that was associated with a subsequent reduced ability to neutralize alpha and beta VoCs.
Keywords: Antibodies; COVID-19; Neutralizing antibodies; Vaccine.
. 2022 Jan 9.
doi: 10.1007/s10875-021-01190-5. Online ahead of print.
Seasonal Betacoronavirus Antibodies' Expansion Post-BNT161b2 Vaccination Associates with Reduced SARS-CoV-2 VoC Neutralization
Stefania Dispinseri[SUP] 1 [/SUP], Ilaria Marzinotto[SUP] 2 [/SUP], Cristina Brigatti[SUP] 2 [/SUP], Maria Franca Pirillo[SUP] 3 [/SUP], Monica Tolazzi[SUP] 1 [/SUP], Elena Bazzigaluppi[SUP] 2 [/SUP], Andrea Canitano[SUP] 3 [/SUP], Martina Borghi[SUP] 4 [/SUP], Alessandra Gallinaro[SUP] 3 [/SUP], Roberta Caccia[SUP] 5 [/SUP], Riccardo Vercesi[SUP] 5 [/SUP], Paul F McKay[SUP] 6 [/SUP], Fabio Ciceri[SUP] 7 8 [/SUP], Lorenzo Piemonti[SUP] 2 7 [/SUP], Donatella Negri[SUP] 4 [/SUP], Paola Cinque[SUP] 5 [/SUP], Andrea Cara[SUP] 3 [/SUP], Gabriella Scarlatti[SUP] 9 [/SUP], Vito Lampasona[SUP] 10 [/SUP]
Affiliations
- PMID: 35000058
- DOI: 10.1007/s10875-021-01190-5
Abstract
SARS-CoV-2 vaccination is known to induce antibodies that recognize also variants of concerns (VoCs) of the virus. However, epidemiological and laboratory evidences indicate that these antibodies have a reduced neutralization ability against VoCs. We studied binding and neutralizing antibodies against the Spike protein domains and subunits of the Wuhan-Hu-1 virus and its alpha, beta, delta VoCs and of seasonal betacoronaviruses (HKU1 and OC43) in a cohort of 31 health care workers prospectively followed post-vaccination with BNT162b2-Comirnaty. The study of sequential samples collected up to 64 days post-vaccination showed that serological assays measuring IgG against Wuhan-Hu-1 antigens were a poor proxy for VoC neutralization. In addition, in subjects who had asymptomatic or mild COVID-19 prior to vaccination, the loss of nAbs following disease could be rapid and accompanied by post-vaccination antibody levels similar to those of naïve vaccinees. Interestingly, in health care workers naïve for SARS-CoV-2 infection, vaccination induced a rapid and transient reactivation of pre-existing seasonal coronaviruses IgG responses that was associated with a subsequent reduced ability to neutralize alpha and beta VoCs.
Keywords: Antibodies; COVID-19; Neutralizing antibodies; Vaccine.
