tetano
Editor, Senior Moderator
J Biomed Nanotechnol
. 2021 Apr 1;17(4):606-614.
doi: 10.1166/jbn.2021.3059.
Development of Antibody-Conjugated Gold and Magnetic Nanocomposites for the Colorimetric Detection of Influenza A Virus
Liqun Ye[SUP] 1 [/SUP], Xiaohong Zhang[SUP] 2 [/SUP], Ningning Yang[SUP] 1 [/SUP], Shunxin Zhao[SUP] 1 [/SUP], Hongmin Wang[SUP] 1 [/SUP], Zhicun Wang[SUP] 3 [/SUP]
Affiliations
Abstract
A novel approach for the detection of influenza virus is of paramount importance for quick diagnosis and therapy. In this study monoclonal antibody (mAb)-conjugated MNPs/AuNPs were developed to detect the H[SUB]1[/SUB]N[SUB]1[/SUB] virus. MNPs and AuNPs were synthesized and loaded with mAbs. The UV-vis spectra exhibited absorbance at 528 nm. XRD revealed the presence of crystalline particles with various diffraction peaks. FTIR confirmed the occurrence of capping molecules in the synthesized NPs. NP stability was evidenced by zeta measurements. The shape and size (mean size 15 nm) of the NPs were determined using SEM and transmission electron microscopy (TEM). In this study the mAb-AuNPs produced a redshift in the absorption spectrum due to plasmon coupling. The absorption increased when H[SUB]1[/SUB]N[SUB]1[/SUB] concentration increased from 0 to 5.0 ng/mL, with the detection limit being 0.05 ng/mL. The sensitivity of mAb-AuNPs was greater than that of ELISA. Since the mAb-AuNP-based colorimetric immunosensor is simple, cost-effective, and rapidly detects H[SUB]1[/SUB]N[SUB]1[/SUB], it has good prospects in pharmaceuticals and clinical diagnosis.
. 2021 Apr 1;17(4):606-614.
doi: 10.1166/jbn.2021.3059.
Development of Antibody-Conjugated Gold and Magnetic Nanocomposites for the Colorimetric Detection of Influenza A Virus
Liqun Ye[SUP] 1 [/SUP], Xiaohong Zhang[SUP] 2 [/SUP], Ningning Yang[SUP] 1 [/SUP], Shunxin Zhao[SUP] 1 [/SUP], Hongmin Wang[SUP] 1 [/SUP], Zhicun Wang[SUP] 3 [/SUP]
Affiliations
- PMID: 35057887
- DOI: 10.1166/jbn.2021.3059
Abstract
A novel approach for the detection of influenza virus is of paramount importance for quick diagnosis and therapy. In this study monoclonal antibody (mAb)-conjugated MNPs/AuNPs were developed to detect the H[SUB]1[/SUB]N[SUB]1[/SUB] virus. MNPs and AuNPs were synthesized and loaded with mAbs. The UV-vis spectra exhibited absorbance at 528 nm. XRD revealed the presence of crystalline particles with various diffraction peaks. FTIR confirmed the occurrence of capping molecules in the synthesized NPs. NP stability was evidenced by zeta measurements. The shape and size (mean size 15 nm) of the NPs were determined using SEM and transmission electron microscopy (TEM). In this study the mAb-AuNPs produced a redshift in the absorption spectrum due to plasmon coupling. The absorption increased when H[SUB]1[/SUB]N[SUB]1[/SUB] concentration increased from 0 to 5.0 ng/mL, with the detection limit being 0.05 ng/mL. The sensitivity of mAb-AuNPs was greater than that of ELISA. Since the mAb-AuNP-based colorimetric immunosensor is simple, cost-effective, and rapidly detects H[SUB]1[/SUB]N[SUB]1[/SUB], it has good prospects in pharmaceuticals and clinical diagnosis.