tetano
Editor, Senior Moderator
J Biomater Appl
. 2022 Jan 7;8853282211063298.
doi: 10.1177/08853282211063298. Online ahead of print.
Lipid-mediated biosynthetic labeling strategy for in vivo dynamic tracing of avian influenza virus infection
Junfang Liu[SUP] 1 [/SUP], Minhong Su[SUP] 1 [/SUP], Xin Chen[SUP] 1 [/SUP], Zhongli Li[SUP] 1 [/SUP], Zekui Fang[SUP] 1 [/SUP], Li Yi[SUP] 2 [/SUP]
Affiliations
Abstract
Monitoring the infection behavior of avian influenza viruses is crucial for understanding viral pathogenesis and preventing its epidemics among people. A number of viral labeling methods have been utilized for tracking viral infection process, but most of them are laborious or decreasing viral activity. Herein we explored a lipid biosynthetic labeling strategy for dynamical tracking the infection of H5N1 pseudotype virus (H5N1p) in host. Biotinylated lipids (biotinyl Cap-PE) were successfully incorporated into viral envelope when it underwent budding process by taking advantage of host cell-derived lipid metabolism. Biotin-H5N1p virus was effectively in situ-labeled with streptavidin-modified near-infrared quantum dots (NIR SA-QDs) using streptavidin-biotin conjugation with well-preserved virus activities. Dual-labeled imaging obviously shows that H5N1p viruses are primarily taken up in host cells via clathrin-mediated endocytosis. In animal models, Virus-conjugated NIR QDs displayed extraordinary photoluminescence, superior stability, and tissue penetration in lung, allowing us to long-term monitor respiratory viral infection in a noninvasive manner. Importantly, the co-localization of viral hemagglutinin protein and QDs in infected lung further conformed the dynamic infection process of virus in vivo. Hence, this in situ QD-labeling strategy based on cell natural biosynthesis provides a brand-new and reliable tool for noninvasion visualizing viral infection in body in a real-time manner.
Keywords: Biosynthetic labeling strategy; avian influenza virus; dynamic visualization; streptavidin-biotin conjugation; viral infection.
. 2022 Jan 7;8853282211063298.
doi: 10.1177/08853282211063298. Online ahead of print.
Lipid-mediated biosynthetic labeling strategy for in vivo dynamic tracing of avian influenza virus infection
Junfang Liu[SUP] 1 [/SUP], Minhong Su[SUP] 1 [/SUP], Xin Chen[SUP] 1 [/SUP], Zhongli Li[SUP] 1 [/SUP], Zekui Fang[SUP] 1 [/SUP], Li Yi[SUP] 2 [/SUP]
Affiliations
- PMID: 34996310
- DOI: 10.1177/08853282211063298
Abstract
Monitoring the infection behavior of avian influenza viruses is crucial for understanding viral pathogenesis and preventing its epidemics among people. A number of viral labeling methods have been utilized for tracking viral infection process, but most of them are laborious or decreasing viral activity. Herein we explored a lipid biosynthetic labeling strategy for dynamical tracking the infection of H5N1 pseudotype virus (H5N1p) in host. Biotinylated lipids (biotinyl Cap-PE) were successfully incorporated into viral envelope when it underwent budding process by taking advantage of host cell-derived lipid metabolism. Biotin-H5N1p virus was effectively in situ-labeled with streptavidin-modified near-infrared quantum dots (NIR SA-QDs) using streptavidin-biotin conjugation with well-preserved virus activities. Dual-labeled imaging obviously shows that H5N1p viruses are primarily taken up in host cells via clathrin-mediated endocytosis. In animal models, Virus-conjugated NIR QDs displayed extraordinary photoluminescence, superior stability, and tissue penetration in lung, allowing us to long-term monitor respiratory viral infection in a noninvasive manner. Importantly, the co-localization of viral hemagglutinin protein and QDs in infected lung further conformed the dynamic infection process of virus in vivo. Hence, this in situ QD-labeling strategy based on cell natural biosynthesis provides a brand-new and reliable tool for noninvasion visualizing viral infection in body in a real-time manner.
Keywords: Biosynthetic labeling strategy; avian influenza virus; dynamic visualization; streptavidin-biotin conjugation; viral infection.