tetano
Editor, Senior Moderator
J Appl Microbiol
. 2022 Dec 15;lxac020.
doi: 10.1093/jambio/lxac020. Online ahead of print.
Efficacy of chemical disinfectants against SARS-CoV-2 on high-touch surface materials
Rachael L Hardison[SUP] 1 [/SUP], Sarah W Nelson[SUP] 1 [/SUP], Rebecca Limmer[SUP] 2 [/SUP], Joel Marx[SUP] 2 [/SUP], Brian M Taylor[SUP] 2 [/SUP], Ryan R James[SUP] 1 [/SUP], Michael J Stewart[SUP] 3 [/SUP], Sang Don D Lee[SUP] 3 [/SUP], Michael Worth Calfee[SUP] 3 [/SUP], Shawn P Ryan[SUP] 3 [/SUP], Megan W Howard[SUP] 1 [/SUP]
Affiliations
Abstract
Aims: This study aimed to provide operationally relevant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surface disinfection efficacy information.
Methods and results: Three EPA-registered disinfectants (Vital Oxide, Peroxide, and Clorox Total 360) and one antimicrobial formulation (CDC bleach) were evaluated against SARS-CoV-2 on material coupons and were tested using Spray (no touch with contact time) and Spray & Wipe (wipe immediately post-application) methods immediately and 2 h post-contamination. Efficacy was evaluated for infectious virus, with a subset tested for viral RNA (vRNA) recovery. Efficacy varied by method, disinfectant, and material. CDC bleach solution showed low efficacy against SARS-CoV-2 (log reduction < 1.7), unless applied via Spray & Wipe. Additionally, mechanical wiping increased the efficacy of treatments against SARS-CoV-2. The recovery of vRNA post-disinfection suggested that vRNA may overestimate infectious virus remaining.
Conclusions: Efficacy depends on surface material, chemical, and disinfection procedure, and suggests that mechanical wiping alone has some efficacy at removing SARS-CoV-2 from surfaces. We observed that disinfectant treatment biased the recovery of vRNA over infectious virus.
Significance and impact of study: These data are useful for developing effective, real-world disinfection procedures, and inform public health experts on the utility of PCR-based surveillance approaches.
Keywords: COVID-19; SARS-CoV-2; disinfection; virucide.
. 2022 Dec 15;lxac020.
doi: 10.1093/jambio/lxac020. Online ahead of print.
Efficacy of chemical disinfectants against SARS-CoV-2 on high-touch surface materials
Rachael L Hardison[SUP] 1 [/SUP], Sarah W Nelson[SUP] 1 [/SUP], Rebecca Limmer[SUP] 2 [/SUP], Joel Marx[SUP] 2 [/SUP], Brian M Taylor[SUP] 2 [/SUP], Ryan R James[SUP] 1 [/SUP], Michael J Stewart[SUP] 3 [/SUP], Sang Don D Lee[SUP] 3 [/SUP], Michael Worth Calfee[SUP] 3 [/SUP], Shawn P Ryan[SUP] 3 [/SUP], Megan W Howard[SUP] 1 [/SUP]
Affiliations
- PMID: 36626793
- DOI: 10.1093/jambio/lxac020
Abstract
Aims: This study aimed to provide operationally relevant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) surface disinfection efficacy information.
Methods and results: Three EPA-registered disinfectants (Vital Oxide, Peroxide, and Clorox Total 360) and one antimicrobial formulation (CDC bleach) were evaluated against SARS-CoV-2 on material coupons and were tested using Spray (no touch with contact time) and Spray & Wipe (wipe immediately post-application) methods immediately and 2 h post-contamination. Efficacy was evaluated for infectious virus, with a subset tested for viral RNA (vRNA) recovery. Efficacy varied by method, disinfectant, and material. CDC bleach solution showed low efficacy against SARS-CoV-2 (log reduction < 1.7), unless applied via Spray & Wipe. Additionally, mechanical wiping increased the efficacy of treatments against SARS-CoV-2. The recovery of vRNA post-disinfection suggested that vRNA may overestimate infectious virus remaining.
Conclusions: Efficacy depends on surface material, chemical, and disinfection procedure, and suggests that mechanical wiping alone has some efficacy at removing SARS-CoV-2 from surfaces. We observed that disinfectant treatment biased the recovery of vRNA over infectious virus.
Significance and impact of study: These data are useful for developing effective, real-world disinfection procedures, and inform public health experts on the utility of PCR-based surveillance approaches.
Keywords: COVID-19; SARS-CoV-2; disinfection; virucide.