tetano
Editor, Senior Moderator
J Vet Sci. 2015 Jan 30. [Epub ahead of print]
[h=1]Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China, 2012 to 2013.[/h] Shen HQ, Yan ZQ, Zeng FG, Liao CT, Zhou QF, Qin JP, Xie QM, Bi YZ, Chen F.
[h=3]Abstract[/h] As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) were isolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes of these strains were sequenced and analyzed. Phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, and the other seven belonged to h9.4.2.6. Specially, we found that all of the viruses isolated in 2013 belonged to lineage h9.4.2.5. The lineage h9.4.2.5 viruses contained a PSRSSR↓GLF motif at HA cleavage site, while the lineage h9.4.2.6 viruses contained a PARSSR↓GLF at the same position. Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200-202, and had an additional one at residues 295-297 in HA1. It is notable that all of the 19 isolates had an amino acid exchange (Q226L) in the receptor binding site, which indicated that the viruses had potential affinity of binding to human like receptor. The present study shows the importance of continuing surveillance of new H9N2 strains in order to better prepare for the next epidemic or pandemic outbreaks of H9N2 AIV infections in chicken flocks.
[h=4]KEYWORDS:[/h] H9N2 subtype; South China; avian influenza virus; phylogenetic analysis
PMID: 25643797 [PubMed - as supplied by publisher]
[h=1]Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China, 2012 to 2013.[/h] Shen HQ, Yan ZQ, Zeng FG, Liao CT, Zhou QF, Qin JP, Xie QM, Bi YZ, Chen F.
[h=3]Abstract[/h] As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) were isolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes of these strains were sequenced and analyzed. Phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, and the other seven belonged to h9.4.2.6. Specially, we found that all of the viruses isolated in 2013 belonged to lineage h9.4.2.5. The lineage h9.4.2.5 viruses contained a PSRSSR↓GLF motif at HA cleavage site, while the lineage h9.4.2.6 viruses contained a PARSSR↓GLF at the same position. Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200-202, and had an additional one at residues 295-297 in HA1. It is notable that all of the 19 isolates had an amino acid exchange (Q226L) in the receptor binding site, which indicated that the viruses had potential affinity of binding to human like receptor. The present study shows the importance of continuing surveillance of new H9N2 strains in order to better prepare for the next epidemic or pandemic outbreaks of H9N2 AIV infections in chicken flocks.
[h=4]KEYWORDS:[/h] H9N2 subtype; South China; avian influenza virus; phylogenetic analysis
PMID: 25643797 [PubMed - as supplied by publisher]