tetano
Editor, Senior Moderator
ISME J
. 2023 Jan 23.
doi: 10.1038/s41396-023-01368-2. Online ahead of print.
Comparative susceptibility of SARS-CoV-2, SARS-CoV, and MERS-CoV across mammals
Meng Li[SUP] #[/SUP][SUP] 1 [/SUP], Juan Du[SUP] #[/SUP][SUP] 1 2 [/SUP], Weiqiang Liu[SUP] 1 2 [/SUP], Zihao Li[SUP] 1 2 [/SUP], Fei Lv[SUP] 1 2 [/SUP], Chunyan Hu[SUP] 1 2 [/SUP], Yichen Dai[SUP] 1 [/SUP], Xiaoxiao Zhang[SUP] 1 2 [/SUP], Zhan Zhang[SUP] 1 2 [/SUP], Gaoming Liu[SUP] 1 [/SUP], Qi Pan[SUP] 1 2 [/SUP], Yang Yu[SUP] 3 [/SUP], Xiao Wang[SUP] 1 [/SUP], Pingfen Zhu[SUP] 1 [/SUP], Xu Tan[SUP] 4 [/SUP], Paul A Garber[SUP] 5 [/SUP], Xuming Zhou[SUP] 6 [/SUP]
Affiliations
Abstract
Exploring wild reservoirs of pathogenic viruses is critical for their long-term control and for predicting future pandemic scenarios. Here, a comparative in vitro infection analysis was first performed on 83 cell cultures derived from 55 mammalian species using pseudotyped viruses bearing S proteins from SARS-CoV-2, SARS-CoV, and MERS-CoV. Cell cultures from Thomas's horseshoe bats, king horseshoe bats, green monkeys, and ferrets were found to be highly susceptible to SARS-CoV-2, SARS-CoV, and MERS-CoV pseudotyped viruses. Moreover, five variants (del69-70, D80Y, S98F, T572I, and Q675H), that beside spike receptor-binding domain can significantly alter the host tropism of SARS-CoV-2. An examination of phylogenetic signals of transduction rates revealed that closely related taxa generally have similar susceptibility to MERS-CoV but not to SARS-CoV and SARS-CoV-2 pseudotyped viruses. Additionally, we discovered that the expression of 95 genes, e.g., PZDK1 and APOBEC3, were commonly associated with the transduction rates of SARS-CoV, MERS-CoV, and SARS-CoV-2 pseudotyped viruses. This study provides basic documentation of the susceptibility, variants, and molecules that underlie the cross-species transmission of these coronaviruses.
. 2023 Jan 23.
doi: 10.1038/s41396-023-01368-2. Online ahead of print.
Comparative susceptibility of SARS-CoV-2, SARS-CoV, and MERS-CoV across mammals
Meng Li[SUP] #[/SUP][SUP] 1 [/SUP], Juan Du[SUP] #[/SUP][SUP] 1 2 [/SUP], Weiqiang Liu[SUP] 1 2 [/SUP], Zihao Li[SUP] 1 2 [/SUP], Fei Lv[SUP] 1 2 [/SUP], Chunyan Hu[SUP] 1 2 [/SUP], Yichen Dai[SUP] 1 [/SUP], Xiaoxiao Zhang[SUP] 1 2 [/SUP], Zhan Zhang[SUP] 1 2 [/SUP], Gaoming Liu[SUP] 1 [/SUP], Qi Pan[SUP] 1 2 [/SUP], Yang Yu[SUP] 3 [/SUP], Xiao Wang[SUP] 1 [/SUP], Pingfen Zhu[SUP] 1 [/SUP], Xu Tan[SUP] 4 [/SUP], Paul A Garber[SUP] 5 [/SUP], Xuming Zhou[SUP] 6 [/SUP]
Affiliations
- PMID: 36690780
- DOI: 10.1038/s41396-023-01368-2
Abstract
Exploring wild reservoirs of pathogenic viruses is critical for their long-term control and for predicting future pandemic scenarios. Here, a comparative in vitro infection analysis was first performed on 83 cell cultures derived from 55 mammalian species using pseudotyped viruses bearing S proteins from SARS-CoV-2, SARS-CoV, and MERS-CoV. Cell cultures from Thomas's horseshoe bats, king horseshoe bats, green monkeys, and ferrets were found to be highly susceptible to SARS-CoV-2, SARS-CoV, and MERS-CoV pseudotyped viruses. Moreover, five variants (del69-70, D80Y, S98F, T572I, and Q675H), that beside spike receptor-binding domain can significantly alter the host tropism of SARS-CoV-2. An examination of phylogenetic signals of transduction rates revealed that closely related taxa generally have similar susceptibility to MERS-CoV but not to SARS-CoV and SARS-CoV-2 pseudotyped viruses. Additionally, we discovered that the expression of 95 genes, e.g., PZDK1 and APOBEC3, were commonly associated with the transduction rates of SARS-CoV, MERS-CoV, and SARS-CoV-2 pseudotyped viruses. This study provides basic documentation of the susceptibility, variants, and molecules that underlie the cross-species transmission of these coronaviruses.