tetano
Editor, Senior Moderator
iScience
. 2021 Aug 4;102941.
doi: 10.1016/j.isci.2021.102941. Online ahead of print.
Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2
Jun-Guy Park[SUP] 1 [/SUP], Fatai S Oladunni[SUP] 1 [/SUP], Mohammed A Rohaim[SUP] 2 3 [/SUP], Jayde Whittingham-Dowd[SUP] 2 [/SUP], James Tollitt[SUP] 2 [/SUP], Matthew D J Hodges[SUP] 2 [/SUP], Nadin Fathallah[SUP] 2 [/SUP], Muhsref Bakri Assas[SUP] 4 [/SUP], Wafaa Alhazmi[SUP] 5 [/SUP], Abdullah Almilaibary[SUP] 6 [/SUP], Munir Iqbal[SUP] 7 [/SUP], Pengxiang Chang[SUP] 7 [/SUP], Renee Escalona[SUP] 1 [/SUP], Vinay Shivanna[SUP] 1 [/SUP], Jordi B Torrelles[SUP] 1 [/SUP], John J Worthington[SUP] 2 [/SUP], Lucy H Jackson-Jones[SUP] 2 [/SUP], Luis Martinez-Sobrido[SUP] 1 [/SUP], Muhammad Munir[SUP] 2 [/SUP]
Affiliations
Abstract
Global deployment of an effective and safe vaccine is necessary to curtail the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy against SARS-CoV-2. Intranasal administration of recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 to mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters immunised with two doses of vaccine showed complete protection from lung infection, inflammation, and pathological lesions following SARS-CoV-2 challenge. Importantly, administration of two doses of intranasal rNDV-S vaccine significantly reduced the SARS-CoV-2 shedding in nasal turbinate and lungs in hamsters. Collectively, intranasal vaccination has the potential to control infection at the site of inoculation, which should prevent both clinical disease and virus transmission to halt the spread of the COVID-19 pandemic.
Keywords: COVID-19; Immunity; Pandemic; SARS-CoV-2; Vaccines; Viral Infection.
. 2021 Aug 4;102941.
doi: 10.1016/j.isci.2021.102941. Online ahead of print.
Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2
Jun-Guy Park[SUP] 1 [/SUP], Fatai S Oladunni[SUP] 1 [/SUP], Mohammed A Rohaim[SUP] 2 3 [/SUP], Jayde Whittingham-Dowd[SUP] 2 [/SUP], James Tollitt[SUP] 2 [/SUP], Matthew D J Hodges[SUP] 2 [/SUP], Nadin Fathallah[SUP] 2 [/SUP], Muhsref Bakri Assas[SUP] 4 [/SUP], Wafaa Alhazmi[SUP] 5 [/SUP], Abdullah Almilaibary[SUP] 6 [/SUP], Munir Iqbal[SUP] 7 [/SUP], Pengxiang Chang[SUP] 7 [/SUP], Renee Escalona[SUP] 1 [/SUP], Vinay Shivanna[SUP] 1 [/SUP], Jordi B Torrelles[SUP] 1 [/SUP], John J Worthington[SUP] 2 [/SUP], Lucy H Jackson-Jones[SUP] 2 [/SUP], Luis Martinez-Sobrido[SUP] 1 [/SUP], Muhammad Munir[SUP] 2 [/SUP]
Affiliations
- PMID: 34368648
- PMCID: PMC8332743
- DOI: 10.1016/j.isci.2021.102941
Abstract
Global deployment of an effective and safe vaccine is necessary to curtail the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety and protective efficacy against SARS-CoV-2. Intranasal administration of recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 to mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T cell-mediated immunity. Hamsters immunised with two doses of vaccine showed complete protection from lung infection, inflammation, and pathological lesions following SARS-CoV-2 challenge. Importantly, administration of two doses of intranasal rNDV-S vaccine significantly reduced the SARS-CoV-2 shedding in nasal turbinate and lungs in hamsters. Collectively, intranasal vaccination has the potential to control infection at the site of inoculation, which should prevent both clinical disease and virus transmission to halt the spread of the COVID-19 pandemic.
Keywords: COVID-19; Immunity; Pandemic; SARS-CoV-2; Vaccines; Viral Infection.