tetano
Editor, Senior Moderator
iScience
. 2023 Sep 22;26(10):108009.
doi: 10.1016/j.isci.2023.108009. eCollection 2023 Oct 20. Broad SARS-CoV-2 neutralization by monoclonal and bispecific antibodies derived from a Gamma-infected individual
Denise Guerra[SUP] 1 2 [/SUP], Tim Beaumont[SUP] 1 2 [/SUP], Laura Radić[SUP] 1 2 [/SUP], Gius Kerster[SUP] 1 2 [/SUP], Karlijn van der Straten[SUP] 1 2 3 [/SUP], Meng Yuan[SUP] 4 [/SUP], Jonathan L Torres[SUP] 4 [/SUP], Wen-Hsin Lee[SUP] 4 [/SUP], Hejun Liu[SUP] 4 [/SUP], Meliawati Poniman[SUP] 1 2 [/SUP], Ilja Bontjer[SUP] 1 2 [/SUP], Judith A Burger[SUP] 1 2 [/SUP], Mathieu Claireaux[SUP] 1 2 [/SUP], Tom G Caniels[SUP] 1 2 [/SUP], Jonne L Snitselaar[SUP] 1 2 [/SUP], Tom P L Bijl[SUP] 1 2 [/SUP], Sabine Kruijer[SUP] 1 2 [/SUP], Gabriel Ozorowski[SUP] 4 [/SUP], David Gideonse[SUP] 5 [/SUP], Kwinten Sliepen[SUP] 1 2 [/SUP], Andrew B Ward[SUP] 4 [/SUP], Dirk Eggink[SUP] 1 2 5 [/SUP], Godelieve J de Bree[SUP] 2 3 [/SUP], Ian A Wilson[SUP] 4 6 [/SUP], Rogier W Sanders[SUP] 1 2 7 [/SUP], Marit J van Gils[SUP] 1 2 [/SUP]
Affiliations
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has remained a medical threat due to the evolution of multiple variants that acquire resistance to vaccines and prior infection. Therefore, it is imperative to discover monoclonal antibodies (mAbs) that neutralize a broad range of SARS-CoV-2 variants. A stabilized spike glycoprotein was used to enrich antigen-specific B cells from an individual with a primary Gamma variant infection. Five mAbs selected from those B cells showed considerable neutralizing potency against multiple variants, with COVA309-35 being the most potent against the autologous virus, as well as Omicron BA.1 and BA.2, and COVA309-22 having binding and neutralization activity against Omicron BA.4/5, BQ.1.1, and XBB.1. When combining the COVA309 mAbs as cocktails or bispecific antibodies, the breadth and potency were improved. In addition, the mechanism of cross-neutralization of the COVA309 mAbs was elucidated by structural analysis. Altogether these data indicate that a Gamma-infected individual can develop broadly neutralizing antibodies.
Keywords: Immunology; Structural biology; Virology.
. 2023 Sep 22;26(10):108009.
doi: 10.1016/j.isci.2023.108009. eCollection 2023 Oct 20. Broad SARS-CoV-2 neutralization by monoclonal and bispecific antibodies derived from a Gamma-infected individual
Denise Guerra[SUP] 1 2 [/SUP], Tim Beaumont[SUP] 1 2 [/SUP], Laura Radić[SUP] 1 2 [/SUP], Gius Kerster[SUP] 1 2 [/SUP], Karlijn van der Straten[SUP] 1 2 3 [/SUP], Meng Yuan[SUP] 4 [/SUP], Jonathan L Torres[SUP] 4 [/SUP], Wen-Hsin Lee[SUP] 4 [/SUP], Hejun Liu[SUP] 4 [/SUP], Meliawati Poniman[SUP] 1 2 [/SUP], Ilja Bontjer[SUP] 1 2 [/SUP], Judith A Burger[SUP] 1 2 [/SUP], Mathieu Claireaux[SUP] 1 2 [/SUP], Tom G Caniels[SUP] 1 2 [/SUP], Jonne L Snitselaar[SUP] 1 2 [/SUP], Tom P L Bijl[SUP] 1 2 [/SUP], Sabine Kruijer[SUP] 1 2 [/SUP], Gabriel Ozorowski[SUP] 4 [/SUP], David Gideonse[SUP] 5 [/SUP], Kwinten Sliepen[SUP] 1 2 [/SUP], Andrew B Ward[SUP] 4 [/SUP], Dirk Eggink[SUP] 1 2 5 [/SUP], Godelieve J de Bree[SUP] 2 3 [/SUP], Ian A Wilson[SUP] 4 6 [/SUP], Rogier W Sanders[SUP] 1 2 7 [/SUP], Marit J van Gils[SUP] 1 2 [/SUP]
Affiliations
- PMID: 37841584
- PMCID: PMC10570122
- DOI: 10.1016/j.isci.2023.108009
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has remained a medical threat due to the evolution of multiple variants that acquire resistance to vaccines and prior infection. Therefore, it is imperative to discover monoclonal antibodies (mAbs) that neutralize a broad range of SARS-CoV-2 variants. A stabilized spike glycoprotein was used to enrich antigen-specific B cells from an individual with a primary Gamma variant infection. Five mAbs selected from those B cells showed considerable neutralizing potency against multiple variants, with COVA309-35 being the most potent against the autologous virus, as well as Omicron BA.1 and BA.2, and COVA309-22 having binding and neutralization activity against Omicron BA.4/5, BQ.1.1, and XBB.1. When combining the COVA309 mAbs as cocktails or bispecific antibodies, the breadth and potency were improved. In addition, the mechanism of cross-neutralization of the COVA309 mAbs was elucidated by structural analysis. Altogether these data indicate that a Gamma-infected individual can develop broadly neutralizing antibodies.
Keywords: Immunology; Structural biology; Virology.