tetano
Editor, Senior Moderator
Int J Infect Dis. 2016 Jan 11. pii: S1201-9712(16)00003-5. doi: 10.1016/j.ijid.2016.01.002. [Epub ahead of print]
[h=1]Is HPAIV H5N1 Fragment Recombined in PB1 the Key for the Epidemic of the novel AIV H7N9 in China, 2013?[/h] Chen L[SUP]1[/SUP], Sun L[SUP]2[/SUP], Li R[SUP]2[/SUP], Chen Y[SUP]3[/SUP], Zhang Z[SUP]4[/SUP], Xiong C[SUP]5[/SUP], Zhao G[SUP]6[/SUP], Jiang Q[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] A novel avian influenza A H7N9 virus that infects human beings was identified in China in 2013. This study is the first time to comprehensively investigate the characteristics of genomic recombination, rather than previously reported reassortment studies.
[h=4]METHODS:[/h] The novel AIV H7N9 genome sequences from NCBI Influenza Virus Sequence Database and the Global Initiative on Sharing Avian Influenza Database (GISAID) were obtained and a representative isolate was determined by homogeneity analysis and phylogenetic tree. Eight segments of the isolate were analyzed to identify segments with recombination events, corresponding recombination fragments and breakpoints. The evolution history of the recombined fragments was tracked by constructing phylogenetic trees of the recombination fragments.
[h=4]RESULTS:[/h] Among the eight segments of the novel AIV H7N9, only PB1 segment showed a marked recombinant phenomenon with 11 recombination events, including five actual recombination and six possible misalignment artifact recombination events. The most notable one was the recombination of a 291nt fragment on the site 490∼780nt that was affiliated to an HPAIV H5N1 (A/tree sparrow/Thailand/VSMU-16-RBR/2005). Phylogenetic tree of the 291nt recombination fragment on the PB1 segment displayed that the novel AIV H7N9 had a close genetic relationship to H9N2 and H5N1.
[h=4]CONCLUSIONS:[/h] The novel AIV H7N9 might reassort its PB1 segment from H9N2 circulated in China, whereas this H9N2 PB1 might be recombined into a highly pathogenic fragment from HPAIV H5N1, which could be the reason for the high fatality rate among patients with AIV H7N9 influenza.
Copyright ? 2016. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] China; H7N9; influenza virus; recombination
PMID: 26778522 [PubMed - as supplied by publisher] Free full text
[h=1]Is HPAIV H5N1 Fragment Recombined in PB1 the Key for the Epidemic of the novel AIV H7N9 in China, 2013?[/h] Chen L[SUP]1[/SUP], Sun L[SUP]2[/SUP], Li R[SUP]2[/SUP], Chen Y[SUP]3[/SUP], Zhang Z[SUP]4[/SUP], Xiong C[SUP]5[/SUP], Zhao G[SUP]6[/SUP], Jiang Q[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] A novel avian influenza A H7N9 virus that infects human beings was identified in China in 2013. This study is the first time to comprehensively investigate the characteristics of genomic recombination, rather than previously reported reassortment studies.
[h=4]METHODS:[/h] The novel AIV H7N9 genome sequences from NCBI Influenza Virus Sequence Database and the Global Initiative on Sharing Avian Influenza Database (GISAID) were obtained and a representative isolate was determined by homogeneity analysis and phylogenetic tree. Eight segments of the isolate were analyzed to identify segments with recombination events, corresponding recombination fragments and breakpoints. The evolution history of the recombined fragments was tracked by constructing phylogenetic trees of the recombination fragments.
[h=4]RESULTS:[/h] Among the eight segments of the novel AIV H7N9, only PB1 segment showed a marked recombinant phenomenon with 11 recombination events, including five actual recombination and six possible misalignment artifact recombination events. The most notable one was the recombination of a 291nt fragment on the site 490∼780nt that was affiliated to an HPAIV H5N1 (A/tree sparrow/Thailand/VSMU-16-RBR/2005). Phylogenetic tree of the 291nt recombination fragment on the PB1 segment displayed that the novel AIV H7N9 had a close genetic relationship to H9N2 and H5N1.
[h=4]CONCLUSIONS:[/h] The novel AIV H7N9 might reassort its PB1 segment from H9N2 circulated in China, whereas this H9N2 PB1 might be recombined into a highly pathogenic fragment from HPAIV H5N1, which could be the reason for the high fatality rate among patients with AIV H7N9 influenza.
Copyright ? 2016. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] China; H7N9; influenza virus; recombination
PMID: 26778522 [PubMed - as supplied by publisher] Free full text