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IOC identifies Aedes aegypti naturally infected with Zika virus for the first time in Brazil

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[h=4]Translation Google

IOC identifies Aedes aegypti naturally infected Zika virus for the first time in Brazil
[/h] Unpublished study indicated the presence of the virus in mosquitoes collected in two locations of Rio de Janeiro. Discovery reinforces the A. aegypti should be the route of more frequent transmission of the virus in Brazil

The Instituto Oswaldo Cruz (IOC / Fiocruz) met for the first time in Brazil, mosquitoes Aedes aegyptinaturally infected with Zika virus. The unprecedented identification occurred during studies of surveillance and monitoring conducted by Mosquitos Laboratory hematozoa Transmitters IOC, which has been collecting mosquitoes in places where they were identified cases of Zika in the state of Rio de Janeiro. Until then, throughout the Americas, there was only a recent report on a set of mosquitoes Aedes albopictus naturally infected Zika virus, made ​​by the Ministry of Health of Mexico.The discovery of the presence of virus in mosquitoes that reinforces A. aegypti should be more frequent route of transmission Zika in Brazil. Data on the finding are in the process of publication.

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The mosquito capture job in the search for identification of circulating viruses is carried out in the surroundings and in the homes of patients with symptoms of Zika, through a partnership with medical Patricia Brazil, researcher at the National Institute of Infectious Diseases Evandro Chagas (INI / Fiocruz ), and with the City of Rio de Janeiro. Over ten months, about 1,500 adult mosquitoes of different species - including males and females - were captured by means of suction equipment and taken to the laboratory analysis. Of these, almost half were of the species A. aegypti . The diagnostic of the presence of Zika virus were performed from two methodologies: molecular analysis by RT-PCR in real time, able to identify the presence of genetic material (RNA) virus, and isolation technique viral, demonstrating that the mosquito carreava infectious viral particles. The results showed the presence of virus in three sets of mosquitoes A. aegypti collected on Rocha Coelho neighborhoods in S?o Jo?o de Meriti, in the Baixada Fluminense, and Realengo, western Rio Zone. Among the mosquitoes caught, no other species was infected.
According to Ricardo Louren?o, head of Mosquitos Laboratory hematozoa Transmitters IOC and leader of the study, to determine the transmission of a disease is carried by a particular species of insect vector is necessary to make two observations. The first is to identify the occurrence of this species which are naturally infected in the field, preferably by virus isolation technique in the laboratory. The second is to prove that this vector is capable of transmitting the virus through tests that assess the ability of transmission. For this, the artificial infection of mosquitoes in a laboratory is performed to verify whether there is the presence of active viral particles in the mosquito's saliva - ie viral particles capable of causing infection.
"Our laboratory scientifically found these two issues, which are fundamental to understand the transmission of the virus, estimate the risk of spread of the disease and direct control actions," he said, adding that the laboratory continues with the collection and analysis activities of mosquitoes.Thus, the discovery of Aedes aegypti naturally infected complements a discovery, published in March 2016, which had shown the vector competence of Brazilian mosquitoes to transmit the Zika virus.The previous study was conducted in partnership with scientists from the Pasteur Institute, France, and published in the international journal 'Plos Neglected Tropical Diseases'. The results showed thatA. aegypti Brazilian can transmit the virus, despite having relatively low vector competence. [Click here to read]
Ricardo Louren?o, factors like behavior, distribution and population density of Aedes in the Brazilian regions may have contributed to the rapid spread of Zika. "In practice, the percentage of A. aegyptiinfected with virus and transmitting diseases is very small. On the other hand, Aedes is a domestic mosquito, who lives next to the man , and benefits from the supply of breeding inside the houses where you can lay their eggs. The large population of mosquitoes and the proximity to humans available to poke and get the blood needed for the reproductive process, added to the large supply of breeding which lay their eggs make the longer alive mosquito, favoring the process of infection and spread of diseases such as dengue, chikungunya and Zika, "he said.
Report: Cristiane Albuquerque
05.23.2016
Permissible playing nonprofit text purposes as long as the source (Communication / Instituto Oswaldo Cruz)


http://www.fiocruz.br/ioc/cgi/cgilua.exe/sys/start.htm?infoid=2560&sid=32
 
Top mosquito suspect found infected with Zika

By Gretchen VogelMay. 23, 2016 , 5:15 PM

The usual suspect has been caught, not red-handed but red-bellied. Since the beginning of the Zika virus outbreak in Brazil, health authorities and researchers have strongly suspected that the mosquito Aedes aegypti, known for spreading several deadly viruses, was also guilty of spreading Zika from one person to another. But direct evidence had been hard to find. Now, researchers in Rio de Janeiro, Brazil, report that they have found the Zika virus in wild-caught A. aegypti. The researchers did not find the virus in other mosquito species they captured in neighborhoods where Zika was spreading, which strengthens the case that A. aegypti is the main vector driving the outbreak.
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http://www.sciencemag.org/news/2016/05/top-mosquito-suspect-found-infected-zika
 
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