tetano
Editor, Senior Moderator
Microbes Infect. 2014 Oct 2. pii: S1286-4579(14)00214-7. doi: 10.1016/j.micinf.2014.09.007. [Epub ahead of print]
Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A(H10N8) in Nanchang City, China.
Ni X1, He F2, Hu M1, Zhou X1, Wang B1, Feng C1, Wu Y3, Li Y4, Tu J1, Li H1, Liu M1, Chen H1, Chen S5.
Author information
Abstract
Multiple reassortment events within poultry and wild birds had resulted in the establishment of another novel avian influenza A(H10N8) virus, and finally resulted in human death in Nanchang, China. However, there was a paucity of information on the prevalence of avian influenza virus in poultry and wild birds in Nanchang area. We investigated avian influenza virus in poultry and wild birds from live poultry markets, poultry countyards, delivery vehicles, and wild-bird habitats in Nanchang. We analyzed 1,036 samples from wild birds and domestic poultry collected from December 2013 to February 2014. Original biological samples were tested for the presence of avian influenza virus using specific primer and probe sets of H5, H7, H9, H10 and N8 subtypes by real-time RT-PCR. In our analysis, the majority(97.98%) of positive samples were from live poultry markets. Among the poultry samples from chickens and ducks, AIV prevalence was 26.05 and 30.81%, respectively. Mixed infection of different HA subtypes was very common. Additionally, H10 subtypes coexistence with N8 was the most prevalent agent during the emergence of H10N8. This event illustrated a long-term surveillance was so helpful for pandemic preparedness and response.
Copyright ? 2014. Published by Elsevier Masson SAS.
KEYWORDS:
Avian flu; Avian influenza virus; H10N8; H7N9
PMID:
25284683
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25284683
Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A(H10N8) in Nanchang City, China.
Ni X1, He F2, Hu M1, Zhou X1, Wang B1, Feng C1, Wu Y3, Li Y4, Tu J1, Li H1, Liu M1, Chen H1, Chen S5.
Author information
Abstract
Multiple reassortment events within poultry and wild birds had resulted in the establishment of another novel avian influenza A(H10N8) virus, and finally resulted in human death in Nanchang, China. However, there was a paucity of information on the prevalence of avian influenza virus in poultry and wild birds in Nanchang area. We investigated avian influenza virus in poultry and wild birds from live poultry markets, poultry countyards, delivery vehicles, and wild-bird habitats in Nanchang. We analyzed 1,036 samples from wild birds and domestic poultry collected from December 2013 to February 2014. Original biological samples were tested for the presence of avian influenza virus using specific primer and probe sets of H5, H7, H9, H10 and N8 subtypes by real-time RT-PCR. In our analysis, the majority(97.98%) of positive samples were from live poultry markets. Among the poultry samples from chickens and ducks, AIV prevalence was 26.05 and 30.81%, respectively. Mixed infection of different HA subtypes was very common. Additionally, H10 subtypes coexistence with N8 was the most prevalent agent during the emergence of H10N8. This event illustrated a long-term surveillance was so helpful for pandemic preparedness and response.
Copyright ? 2014. Published by Elsevier Masson SAS.
KEYWORDS:
Avian flu; Avian influenza virus; H10N8; H7N9
PMID:
25284683
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25284683