tetano
Editor, Senior Moderator
J Ethnopharmacol. 2019 Oct 20:112311. doi: 10.1016/j.jep.2019.112311. [Epub ahead of print] [h=1]Inula helenium and Grindelia squarrosa as a source of compounds with anti-inflammatory activity in human neutrophils and cultured human respiratory epithelium.[/h]
Michalak B[SUP]1[/SUP], Gierlikowski W[SUP]2[/SUP], Bekier K[SUP]3[/SUP], Skalicka-Woźniak K[SUP]4[/SUP], Czerwińska ME[SUP]3[/SUP], Kiss AK[SUP]5[/SUP].
[h=3]Author information[/h] 1 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. Electronic address: bmichalak@wum.edu.pl. 2 Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Banacha 1a, 02-097, Warsaw, Poland; Genomic Medicine, Medical University of Warsaw, Nielubowicza 5, 02-097, Warsaw, Poland. 3 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. 4 Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki 1, 20-093, Lublin, Poland. 5 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. Electronic address: akiss@wum.edu.pl.
[h=3]Abstract[/h] [h=4]ETHNOPHARMACOLOGICAL RELEVANCE:[/h] During the epidemic season, over 90% of acute wheezing disease is associated with bronchial inflammation. Both neutrophil- and eosinophil-mediated inflammation have been involved in the pathophysiology of acute bronchitis, but neutrophil cell recruitment has been shown to be dominant. The ongoing inflammation increase the chemotaxis of neutrophils to inflamed site providing to their overaccumulation. The pharmacological reduction of neutrophil migration can be limited by suppression of major chemoattractants (IL-8, IL-1β and TNF-α) release and downregulation of adhesive molecules.
[h=4]AIM OF THE STUDY:[/h] During a screening of plants traditionally used in respiratory tracts diseases (e.g. cough, rhinitis, bronchitis, throat infection, fever, influenza) in Europe, we have selected roots of Inula helenium and aerial parts of Grindelia squarrosa as a potential source of compounds limiting neutrophil migration.
[h=4]MATERIALS AND METHODS:[/h] The effect on IL-8, IL-1β and TNF-α release by neutrophils and respiratory epithelium cell line (A549) was measured by enzyme-linked immunosorbent assay (ELISA). The surface expression of adhesive molecules was analyzed with flow cytometry, and the neutrophil attachment to the epithelial cells was assessed fluorimetrically.
[h=4]RESULTS:[/h] We confirmed the ability of selected extracts and compounds to suppress neutrophil binding to the epithelium surface via downregulation of β2 integrin. Compared with a positive controls, budesonide and clarithromycin selected compounds, alantolactone and grindelic acid have been shown a comparable inhibition of proinflammatory cytokines (IL-8, TNF-α, IL-1β).
[h=4]CONCLUSIONS:[/h] The present study demonstrated that the Inula helenium and Grindelia squarrosa, which have been traditionally used in Europe as medicinal plants, are a valuable source of active compounds with anti-inflammatory activity. Our observations justify the traditional use of I. helenium and G. squarrosa for a treatment inflammation-based diseases in respiratory tract.
Copyright ? 2019. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Grindelia squarrosa; Human respiratory epithelium; Interleukin 8; Inula helenium; Neutrophils; Pro-inflammatory mediators
PMID: 31644941 DOI: 10.1016/j.jep.2019.112311
Michalak B[SUP]1[/SUP], Gierlikowski W[SUP]2[/SUP], Bekier K[SUP]3[/SUP], Skalicka-Woźniak K[SUP]4[/SUP], Czerwińska ME[SUP]3[/SUP], Kiss AK[SUP]5[/SUP].
[h=3]Author information[/h] 1 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. Electronic address: bmichalak@wum.edu.pl. 2 Department of Internal Medicine and Endocrinology, Medical University of Warsaw, Banacha 1a, 02-097, Warsaw, Poland; Genomic Medicine, Medical University of Warsaw, Nielubowicza 5, 02-097, Warsaw, Poland. 3 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. 4 Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Chodzki 1, 20-093, Lublin, Poland. 5 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland. Electronic address: akiss@wum.edu.pl.
[h=3]Abstract[/h] [h=4]ETHNOPHARMACOLOGICAL RELEVANCE:[/h] During the epidemic season, over 90% of acute wheezing disease is associated with bronchial inflammation. Both neutrophil- and eosinophil-mediated inflammation have been involved in the pathophysiology of acute bronchitis, but neutrophil cell recruitment has been shown to be dominant. The ongoing inflammation increase the chemotaxis of neutrophils to inflamed site providing to their overaccumulation. The pharmacological reduction of neutrophil migration can be limited by suppression of major chemoattractants (IL-8, IL-1β and TNF-α) release and downregulation of adhesive molecules.
[h=4]AIM OF THE STUDY:[/h] During a screening of plants traditionally used in respiratory tracts diseases (e.g. cough, rhinitis, bronchitis, throat infection, fever, influenza) in Europe, we have selected roots of Inula helenium and aerial parts of Grindelia squarrosa as a potential source of compounds limiting neutrophil migration.
[h=4]MATERIALS AND METHODS:[/h] The effect on IL-8, IL-1β and TNF-α release by neutrophils and respiratory epithelium cell line (A549) was measured by enzyme-linked immunosorbent assay (ELISA). The surface expression of adhesive molecules was analyzed with flow cytometry, and the neutrophil attachment to the epithelial cells was assessed fluorimetrically.
[h=4]RESULTS:[/h] We confirmed the ability of selected extracts and compounds to suppress neutrophil binding to the epithelium surface via downregulation of β2 integrin. Compared with a positive controls, budesonide and clarithromycin selected compounds, alantolactone and grindelic acid have been shown a comparable inhibition of proinflammatory cytokines (IL-8, TNF-α, IL-1β).
[h=4]CONCLUSIONS:[/h] The present study demonstrated that the Inula helenium and Grindelia squarrosa, which have been traditionally used in Europe as medicinal plants, are a valuable source of active compounds with anti-inflammatory activity. Our observations justify the traditional use of I. helenium and G. squarrosa for a treatment inflammation-based diseases in respiratory tract.
Copyright ? 2019. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Grindelia squarrosa; Human respiratory epithelium; Interleukin 8; Inula helenium; Neutrophils; Pro-inflammatory mediators
PMID: 31644941 DOI: 10.1016/j.jep.2019.112311