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Intranasal immunization of guinea pig with trivalent influenza antigen adjuvanted by Cyclamen europaeum tubers extract

tetano

Editor, Senior Moderator
Arch Virol. 2018 Sep 17. doi: 10.1007/s00705-018-4023-3. [Epub ahead of print]
[h=1]Intranasal immunization of guinea pig with trivalent influenza antigen adjuvanted by Cyclamen europaeum tubers extract.[/h] Evseenko VA[SUP]1[/SUP], Kolosova NP[SUP]2[/SUP], Gudymo AS[SUP]2[/SUP], Maltsev SV[SUP]2[/SUP], Bulanovich JA[SUP]2[/SUP], Goncharova NI[SUP]2[/SUP], Danilchenko NV[SUP]2[/SUP], Marchenko VY[SUP]2[/SUP], Susloparov IM[SUP]2[/SUP], Ryzhikov AB[SUP]2[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] Cyclamen europaeum tubers extract (CTE) with concentration commonly used for human rhinosinusitis treatment was tested as mucosal adjuvant in experimental intranasal immunization of guinea pigs with concentrated commercially available influenza trivalent vaccine and subsequent infection with influenza strain A/California/04/2009 H1N1pdm. Dual intranasal immunization with vaccine compound consisting of 7.5 ?g of each hemagglutinin and 500 ?g of CTE in 50 ?l induced reciprocal GMT on day 21 after immunization 40 (5-640) against H1N1pdm; 43.20 (5-1280) against H3N2; 10.80 (5-80) against influenza B. Animals with HI titers 1/80 against cell-derived antigen were completely protected against challenge with A/California/04/2009 H1N1pdm09.


PMID: 30225522 DOI: 10.1007/s00705-018-4023-3
 
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