tetano
Editor, Senior Moderator
Int J Pharm. 2014 Jul 3. pii: S0378-5173(14)00494-3. doi: 10.1016/j.ijpharm.2014.07.005. [Epub ahead of print]
Intranasal chitosan-DNA vaccines that protect across influenza virus subtypes.
Sawaengsak C1, Mori Y2, Yamanishi K3, Srimanote P4, Chaicumpa W5, Mitrevej A6, Sinchaipanid N7.
Author information
Abstract
An egg-free and broadly effective influenza vaccine that can be produced rapidly, adequately and cost-effectively is needed. In this study, chitosan-associated DNAs prepared at various nitrogen/phosphate charge (N/P) ratios were studied for their physicochemical properties, stability, cytotoxicity, and protein expression ability. The chitosan-DNA complexes (chitoplexes) of the N/P ratio 2 had the required characteristics including optimal size range, positive surface charge, high DNA association efficiency, tolerability to DNase digestion and mammalian cell viability compatibility. The N/P ratio 2-chitoplexes revealed the highest green fluorescent protein and luciferase expressions in the transfected mammalian cell cultures and in the mouse lungs. Mice immunized intranasally with the N/P ratio 2-chitoplex vaccines carrying DNAs coding for conserved proteins of influenza virus, i.e., ion channel protein (M2) and/or nucleoprotein (NP), had both mucosal and systemic humoral as well as cell mediated immune responses to the in vivo expressed antigens which conferred protection in mice against the lethal challenges not only with the homologous influenza virus subtype (H1N1), but also the heterologous subtype (H3N2). The chitoplexes should be considered as influenza vaccine candidates especially during the period of high vaccine demand. They are suitable for developing areas of the world where conventional vaccine production capacity is lacking.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Chitosan self assembly; DNA vaccine; Heterosubtypic immunity; Influenza; Intranasal vaccination; Particulate gene delivery system
PMID:
24998507
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24998507
Intranasal chitosan-DNA vaccines that protect across influenza virus subtypes.
Sawaengsak C1, Mori Y2, Yamanishi K3, Srimanote P4, Chaicumpa W5, Mitrevej A6, Sinchaipanid N7.
Author information
Abstract
An egg-free and broadly effective influenza vaccine that can be produced rapidly, adequately and cost-effectively is needed. In this study, chitosan-associated DNAs prepared at various nitrogen/phosphate charge (N/P) ratios were studied for their physicochemical properties, stability, cytotoxicity, and protein expression ability. The chitosan-DNA complexes (chitoplexes) of the N/P ratio 2 had the required characteristics including optimal size range, positive surface charge, high DNA association efficiency, tolerability to DNase digestion and mammalian cell viability compatibility. The N/P ratio 2-chitoplexes revealed the highest green fluorescent protein and luciferase expressions in the transfected mammalian cell cultures and in the mouse lungs. Mice immunized intranasally with the N/P ratio 2-chitoplex vaccines carrying DNAs coding for conserved proteins of influenza virus, i.e., ion channel protein (M2) and/or nucleoprotein (NP), had both mucosal and systemic humoral as well as cell mediated immune responses to the in vivo expressed antigens which conferred protection in mice against the lethal challenges not only with the homologous influenza virus subtype (H1N1), but also the heterologous subtype (H3N2). The chitoplexes should be considered as influenza vaccine candidates especially during the period of high vaccine demand. They are suitable for developing areas of the world where conventional vaccine production capacity is lacking.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Chitosan self assembly; DNA vaccine; Heterosubtypic immunity; Influenza; Intranasal vaccination; Particulate gene delivery system
PMID:
24998507
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24998507