tetano
Editor, Senior Moderator
Cell Host Microbe. 2016 Feb 10;19(2):254-66. doi: 10.1016/j.chom.2016.01.002.
[h=1]Integrated Omics Analysis of Pathogenic Host Responses during Pandemic H1N1 Influenza Virus Infection: The Crucial Role of Lipid Metabolism.[/h] Tisoncik-Go J[SUP]1[/SUP], Gasper DJ[SUP]2[/SUP], Kyle JE[SUP]3[/SUP], Eisfeld AJ[SUP]2[/SUP], Selinger C[SUP]1[/SUP], Hatta M[SUP]2[/SUP], Morrison J[SUP]1[/SUP], Korth MJ[SUP]1[/SUP], Zink EM[SUP]3[/SUP], Kim YM[SUP]3[/SUP], Schepmoes AA[SUP]3[/SUP], Nicora CD[SUP]3[/SUP], Purvine SO[SUP]4[/SUP], Weitz KK[SUP]3[/SUP], Peng X[SUP]1[/SUP], Green RR[SUP]1[/SUP], Tilton SC[SUP]3[/SUP], Webb-Robertson BJ[SUP]5[/SUP], Waters KM[SUP]3[/SUP], Metz TO[SUP]3[/SUP], Smith RD[SUP]3[/SUP], Kawaoka Y[SUP]6[/SUP], Suresh M[SUP]2[/SUP], Josset L[SUP]7[/SUP], Katze MG[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Pandemic influenza viruses modulate proinflammatory responses that can lead to immunopathogenesis. We present an extensive and systematic profiling of lipids, metabolites, and proteins in respiratory compartments of ferrets infected with either 1918 or 2009 human pandemic H1N1 influenza viruses. Integrative analysis of high-throughput omics data with virologic and histopathologic data uncovered relationships between host responses and phenotypic outcomes of viral infection. Proinflammatory lipid precursors in the trachea following 1918 infection correlated with severe tracheal lesions. Using an algorithm to infer cell quantity changes from gene expression data, we found enrichment of distinct T cell subpopulations in the trachea. There was also a predicted increase in inflammatory monocytes in the lung of 1918 virus-infected animals that was sustained throughout infection. This study presents a unique resource to the influenza research community and demonstrates the utility of an integrative systems approach for characterization of lipid metabolism alterations underlying respiratory responses to viruses.
Copyright ? 2016 Elsevier Inc. All rights reserved.
PMID: 26867183 [PubMed - in process]
[h=1]Integrated Omics Analysis of Pathogenic Host Responses during Pandemic H1N1 Influenza Virus Infection: The Crucial Role of Lipid Metabolism.[/h] Tisoncik-Go J[SUP]1[/SUP], Gasper DJ[SUP]2[/SUP], Kyle JE[SUP]3[/SUP], Eisfeld AJ[SUP]2[/SUP], Selinger C[SUP]1[/SUP], Hatta M[SUP]2[/SUP], Morrison J[SUP]1[/SUP], Korth MJ[SUP]1[/SUP], Zink EM[SUP]3[/SUP], Kim YM[SUP]3[/SUP], Schepmoes AA[SUP]3[/SUP], Nicora CD[SUP]3[/SUP], Purvine SO[SUP]4[/SUP], Weitz KK[SUP]3[/SUP], Peng X[SUP]1[/SUP], Green RR[SUP]1[/SUP], Tilton SC[SUP]3[/SUP], Webb-Robertson BJ[SUP]5[/SUP], Waters KM[SUP]3[/SUP], Metz TO[SUP]3[/SUP], Smith RD[SUP]3[/SUP], Kawaoka Y[SUP]6[/SUP], Suresh M[SUP]2[/SUP], Josset L[SUP]7[/SUP], Katze MG[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Pandemic influenza viruses modulate proinflammatory responses that can lead to immunopathogenesis. We present an extensive and systematic profiling of lipids, metabolites, and proteins in respiratory compartments of ferrets infected with either 1918 or 2009 human pandemic H1N1 influenza viruses. Integrative analysis of high-throughput omics data with virologic and histopathologic data uncovered relationships between host responses and phenotypic outcomes of viral infection. Proinflammatory lipid precursors in the trachea following 1918 infection correlated with severe tracheal lesions. Using an algorithm to infer cell quantity changes from gene expression data, we found enrichment of distinct T cell subpopulations in the trachea. There was also a predicted increase in inflammatory monocytes in the lung of 1918 virus-infected animals that was sustained throughout infection. This study presents a unique resource to the influenza research community and demonstrates the utility of an integrative systems approach for characterization of lipid metabolism alterations underlying respiratory responses to viruses.
Copyright ? 2016 Elsevier Inc. All rights reserved.
PMID: 26867183 [PubMed - in process]