tetano
Editor, Senior Moderator
Int J Pharm
. 2021 Aug 17;121024.
doi: 10.1016/j.ijpharm.2021.121024. Online ahead of print.
Assessment of adjuvantation strategy of lipid squalene nanoparticles for enhancing the immunogenicity of a SARS-CoV-2 spike subunit protein against COVID-19
Hui-Min Ho[SUP] 1 [/SUP], Chiung-Yi Huang[SUP] 1 [/SUP], Yu-Jhen Cheng[SUP] 1 [/SUP], Kuan-Yin Shen[SUP] 1 [/SUP], Tsai-Teng Tzeng[SUP] 1 [/SUP], Shih-Jen Liu[SUP] 2 [/SUP], Hsin-Wei Chen[SUP] 2 [/SUP], Chung-Hsiung Huang[SUP] 3 [/SUP], Ming-Hsi Huang[SUP] 4 [/SUP]
Affiliations
Abstract
Vaccination is regarded as the most effective intervention for controlling the coronavirus disease 2019 (COVID-19) pandemic. The objective of this study is to provide comprehensive information on lipid squalene nanoparticle (SQ@NP)-adjuvanted COVID-19 vaccines regarding modulating immune response and enhancing vaccine efficacy. After being adjuvanted with SQ@NP, the SARS-CoV-2 spike (S) subunit protein was intramuscularly (i.m.) administered to mice. Serum samples investigated by ELISA and virus neutralizing assay showed that a single-dose SQ@NP-adjuvanted S-protein vaccine can induce antigen-specific IgG and protective antibodies comparable with those induced by two doses of nonadjuvanted protein vaccine. When the mice received a boosting vaccine injection, anamnestic response was observed in the groups of adjuvanted vaccine. Furthermore, the secretion of cytokines in splenocytes, such as interferon (IFN)-γ, interleukin (IL)-5 and IL-10, was significantly enhanced after adjuvantation of S-protein vaccine with SQ@NP; however, this was not the case for the vaccine adjuvanted with conventional aluminum mineral salts. Histological examination of injection sites showed that the SQ@NP-adjuvanted vaccine was considerably well tolerated following i.m. injection in mice. These results pave the way for the performance tuning of optimal vaccine formulations against COVID-19.
Keywords: COVID-19 vaccine; immunomodulation; lipid squalene nanoparticles; single injection; vaccine adjuvant.
. 2021 Aug 17;121024.
doi: 10.1016/j.ijpharm.2021.121024. Online ahead of print.
Assessment of adjuvantation strategy of lipid squalene nanoparticles for enhancing the immunogenicity of a SARS-CoV-2 spike subunit protein against COVID-19
Hui-Min Ho[SUP] 1 [/SUP], Chiung-Yi Huang[SUP] 1 [/SUP], Yu-Jhen Cheng[SUP] 1 [/SUP], Kuan-Yin Shen[SUP] 1 [/SUP], Tsai-Teng Tzeng[SUP] 1 [/SUP], Shih-Jen Liu[SUP] 2 [/SUP], Hsin-Wei Chen[SUP] 2 [/SUP], Chung-Hsiung Huang[SUP] 3 [/SUP], Ming-Hsi Huang[SUP] 4 [/SUP]
Affiliations
- PMID: 34416331
- DOI: 10.1016/j.ijpharm.2021.121024
Abstract
Vaccination is regarded as the most effective intervention for controlling the coronavirus disease 2019 (COVID-19) pandemic. The objective of this study is to provide comprehensive information on lipid squalene nanoparticle (SQ@NP)-adjuvanted COVID-19 vaccines regarding modulating immune response and enhancing vaccine efficacy. After being adjuvanted with SQ@NP, the SARS-CoV-2 spike (S) subunit protein was intramuscularly (i.m.) administered to mice. Serum samples investigated by ELISA and virus neutralizing assay showed that a single-dose SQ@NP-adjuvanted S-protein vaccine can induce antigen-specific IgG and protective antibodies comparable with those induced by two doses of nonadjuvanted protein vaccine. When the mice received a boosting vaccine injection, anamnestic response was observed in the groups of adjuvanted vaccine. Furthermore, the secretion of cytokines in splenocytes, such as interferon (IFN)-γ, interleukin (IL)-5 and IL-10, was significantly enhanced after adjuvantation of S-protein vaccine with SQ@NP; however, this was not the case for the vaccine adjuvanted with conventional aluminum mineral salts. Histological examination of injection sites showed that the SQ@NP-adjuvanted vaccine was considerably well tolerated following i.m. injection in mice. These results pave the way for the performance tuning of optimal vaccine formulations against COVID-19.
Keywords: COVID-19 vaccine; immunomodulation; lipid squalene nanoparticles; single injection; vaccine adjuvant.