tetano
Editor, Senior Moderator
Int J Med Microbiol
. 2024 Jan 11:314:151598.
doi: 10.1016/j.ijmm.2024.151598. Online ahead of print. Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
Yuguang Fu[SUP] 1 [/SUP], Marianne Wedde[SUP] 2 [/SUP], Sigrun Smola[SUP] 3 [/SUP], Djin-Ye Oh[SUP] 2 [/SUP], Thorsten Pfuhl[SUP] 3 [/SUP], Jürgen Rissland[SUP] 3 [/SUP], Michael Zemlin[SUP] 4 [/SUP], Fidelis A Flockerzi[SUP] 5 [/SUP], Rainer M Bohle[SUP] 5 [/SUP], Andrea Thürmer[SUP] 6 [/SUP], Susanne Duwe[SUP] 2 [/SUP], Barbara Biere[SUP] 2 [/SUP], Janine Reiche[SUP] 2 [/SUP], Brunhilde Schweiger[SUP] 2 [/SUP], Christin Mache[SUP] 2 [/SUP], Thorsten Wolff[SUP] 2 [/SUP], Georg Herrler[SUP] 7 [/SUP], Ralf Dürrwald[SUP] 8 [/SUP]
Affiliations
Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized. A patient (age group 5-15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined. Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed. The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
Keywords: A(H1N1)pdm09 virus; Fatal influenza; S263S/F (HA1) and H456H/Y (PB1) mutations.
. 2024 Jan 11:314:151598.
doi: 10.1016/j.ijmm.2024.151598. Online ahead of print. Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome
Yuguang Fu[SUP] 1 [/SUP], Marianne Wedde[SUP] 2 [/SUP], Sigrun Smola[SUP] 3 [/SUP], Djin-Ye Oh[SUP] 2 [/SUP], Thorsten Pfuhl[SUP] 3 [/SUP], Jürgen Rissland[SUP] 3 [/SUP], Michael Zemlin[SUP] 4 [/SUP], Fidelis A Flockerzi[SUP] 5 [/SUP], Rainer M Bohle[SUP] 5 [/SUP], Andrea Thürmer[SUP] 6 [/SUP], Susanne Duwe[SUP] 2 [/SUP], Barbara Biere[SUP] 2 [/SUP], Janine Reiche[SUP] 2 [/SUP], Brunhilde Schweiger[SUP] 2 [/SUP], Christin Mache[SUP] 2 [/SUP], Thorsten Wolff[SUP] 2 [/SUP], Georg Herrler[SUP] 7 [/SUP], Ralf Dürrwald[SUP] 8 [/SUP]
Affiliations
- PMID: 38237287
- DOI: 10.1016/j.ijmm.2024.151598
Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized. A patient (age group 5-15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well-differentiated airway cells were determined. Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air-liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed. The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.
Keywords: A(H1N1)pdm09 virus; Fatal influenza; S263S/F (HA1) and H456H/Y (PB1) mutations.