tetano
Editor, Senior Moderator
Int J Infect Dis
. 2021 Oct 21;S1201-9712(21)00820-1.
doi: 10.1016/j.ijid.2021.10.033. Online ahead of print.
T cell receptor repertoires as potential diagnostic markers for patients with COVID-19
Xianliang Hou[SUP] 1 [/SUP], Wentao Fan[SUP] 2 [/SUP], Guangyu Wang[SUP] 3 [/SUP], Xiaoyan Chen[SUP] 4 [/SUP], Chune Mo[SUP] 1 [/SUP], Yongsi Wang[SUP] 2 [/SUP], Weiwei Gong[SUP] 1 [/SUP], Xuyan Wen[SUP] 5 [/SUP], Hui Chen[SUP] 2 [/SUP], Dan He[SUP] 2 [/SUP], Lijun Mo[SUP] 6 [/SUP], Shaofeng Jiang[SUP] 7 [/SUP], Minglin Ou[SUP] 8 [/SUP], Haonan Guo[SUP] 9 [/SUP], Hongbo Liu[SUP] 10 [/SUP]
Affiliations
Abstract
Objective: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an ongoing global health emergency. T cell receptor (TCR) are crucial mediators of antiviral adaptive immunity. Here, we sought to comprehensively characterize the TCR repertoire changes in patients with COVID-19.
Design: We implemented a large sample size multi-center randomized controlled trial to study the TCR repertoire features, and identify COVID-19 disease-related TCR sequences.
Results: We found that some TCRβ features of COVID-19 patients were remarkably different from that of healthy control, including decreased repertoire diversity, longer CDR3 length, skewed utilization of the TRBV/J, and a high degree of TCRβ sharing. Moreover, this analysis showed TCR repertoire diversity declines with aging, which may be a cause of higher infection and mortality in elderly patients. Importantly we identified a set of TCRβ clones, which can distinguish COVID-19 patients from healthy controls with high accuracy. Notably, this diagnostic model demonstrates 100% specificity and 82.68% sensitivity at 0-3 days post diagnosis.
Conclusions: This study lay the foundation for immunodiagnosis and the development of medicines and vaccines for COVID-19 patients.
Keywords: Adaptive immunity; Coronavirus disease 2019; SARS-CoV-2; T cell receptor.
. 2021 Oct 21;S1201-9712(21)00820-1.
doi: 10.1016/j.ijid.2021.10.033. Online ahead of print.
T cell receptor repertoires as potential diagnostic markers for patients with COVID-19
Xianliang Hou[SUP] 1 [/SUP], Wentao Fan[SUP] 2 [/SUP], Guangyu Wang[SUP] 3 [/SUP], Xiaoyan Chen[SUP] 4 [/SUP], Chune Mo[SUP] 1 [/SUP], Yongsi Wang[SUP] 2 [/SUP], Weiwei Gong[SUP] 1 [/SUP], Xuyan Wen[SUP] 5 [/SUP], Hui Chen[SUP] 2 [/SUP], Dan He[SUP] 2 [/SUP], Lijun Mo[SUP] 6 [/SUP], Shaofeng Jiang[SUP] 7 [/SUP], Minglin Ou[SUP] 8 [/SUP], Haonan Guo[SUP] 9 [/SUP], Hongbo Liu[SUP] 10 [/SUP]
Affiliations
- PMID: 34688948
- DOI: 10.1016/j.ijid.2021.10.033
Abstract
Objective: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an ongoing global health emergency. T cell receptor (TCR) are crucial mediators of antiviral adaptive immunity. Here, we sought to comprehensively characterize the TCR repertoire changes in patients with COVID-19.
Design: We implemented a large sample size multi-center randomized controlled trial to study the TCR repertoire features, and identify COVID-19 disease-related TCR sequences.
Results: We found that some TCRβ features of COVID-19 patients were remarkably different from that of healthy control, including decreased repertoire diversity, longer CDR3 length, skewed utilization of the TRBV/J, and a high degree of TCRβ sharing. Moreover, this analysis showed TCR repertoire diversity declines with aging, which may be a cause of higher infection and mortality in elderly patients. Importantly we identified a set of TCRβ clones, which can distinguish COVID-19 patients from healthy controls with high accuracy. Notably, this diagnostic model demonstrates 100% specificity and 82.68% sensitivity at 0-3 days post diagnosis.
Conclusions: This study lay the foundation for immunodiagnosis and the development of medicines and vaccines for COVID-19 patients.
Keywords: Adaptive immunity; Coronavirus disease 2019; SARS-CoV-2; T cell receptor.