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Int J Infect Dis . Isolation and phylogenetic analysis of SARS-CoV-2 variants collected in Russia during COVID-19 outbreak

tetano

Editor, Senior Moderator
Int J Infect Dis


. 2020 Jul 25;S1201-9712(20)30566-X.
doi: 10.1016/j.ijid.2020.07.024. Online ahead of print.
Isolation and phylogenetic analysis of SARS-CoV-2 variants collected in Russia during COVID-19 outbreak


Liubov Kozlovskaya[SUP] 1 [/SUP], Anastasia Piniaeva[SUP] 2 [/SUP], Georgy Ignatyev[SUP] 2 [/SUP], Alexey Selivanov[SUP] 2 [/SUP], Anna Shishova[SUP] 3 [/SUP], Anastasia Kovpak[SUP] 2 [/SUP], Ilya Gordeychuk[SUP] 3 [/SUP], Yury Ivin[SUP] 2 [/SUP], Anastasia Berestovskaya[SUP] 4 [/SUP], Egor Prokhortchouk[SUP] 5 [/SUP], Denis Protsenko[SUP] 4 [/SUP], Mikhail Rychev[SUP] 5 [/SUP], Aydar Ishmukhametov[SUP] 3 [/SUP]



Affiliations

Abstract

Objectives: The novel coronavirus outbreak (COVID-19) started in December 2019 in China and in the next months spread all over the world, involving 188 countries. The objective of the present study was an attempt to understand the molecular epidemiology of the COVID-19 outbreak in Russia.
Methods: Here we isolated and genetically characterized two SARS-CoV-2 strains, performed phylogenetic analysis of all available Russian sequences and compared the epidemiological data on COVID-19 incidence to evaluate the molecular epidemiology and virus spread patterns on the territory of Russia.
Results and conclusions: Whole genome analysis of isolates obtained in this study and 216 others isolated in Russia revealed a set of seven common mutations compared to the original Wuhan virus, including amino acid substitutions in spike protein S and nucleoprotein N, possibly affecting their properties. Phylogenetic analysis of all Russian sequences and 8 717 sequences from other countries showed multiple importations of the virus into Russia, local circulation and several patterns of virus spread.

Keywords: COVID-19; Russia; SARS-CoV-2; epidemiology; mutation; phylogenetic analysis.
 
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