tetano
Editor, Senior Moderator
Int J Infect Dis
. 2021 Aug 15;S1201-9712(21)00659-7.
doi: 10.1016/j.ijid.2021.08.026. Online ahead of print.
Cross-reactive humoral immune responses against seasonal human coronaviruses in COVID-19 patients with different disease severity
Kazuo Imai[SUP] 1 [/SUP], Masaru Matsuoka[SUP] 2 [/SUP], Sakiko Tabata[SUP] 3 [/SUP], Yutaro Kitagawa[SUP] 2 [/SUP], Mayu Nagura-Ikeda[SUP] 3 [/SUP], Katsumi Kubota[SUP] 2 [/SUP], Ai Fukada[SUP] 2 [/SUP], Tomohito Takada[SUP] 2 [/SUP], Momoko Sato[SUP] 2 [/SUP], Sakiko Noguchi[SUP] 2 [/SUP], Shinichi Takeuchi[SUP] 2 [/SUP], Noriaki Arakawa[SUP] 4 [/SUP], Kazuyasu Miyoshi[SUP] 3 [/SUP], Yoshiro Saito[SUP] 4 [/SUP], Takuya Maeda[SUP] 2 [/SUP]
Affiliations
Abstract
Background: We evaluated the cross-reactive antibody response against seasonal human coronaviruses (HCoVs) according to disease severity in patients with COVID-19 in Japan.
Methods: We analyzed 194 paired-collected serum samples from 97 patients with COVID-19 (mild, 35; severe, 62) on admission and during convalescence. IgG antibodies against the nucleocapsid (N) and spike (S) proteins of SARS-CoV-2 and four seasonal HCoVs (HCoV-NL63, -229E, -OC43, and -HKU1) were detected by enzyme-linked immunosorbent assays.
Results: There was no difference in optical density (OD) values for seasonal HCoVs on admission between the severe and mild cases. In addition, a specific pattern of disease severity-associated OD values for HCoVs was not identified. A significant increase of OD values from admission to convalescence for HCoV-HKU1and -OC43 IgG-S and HCoV-NL63 and -229E IgG-N was observed in the severe cases. Significant differences were observed between the mild and severe cases for HCoV-HKU1 and -OC43 IgG-S OD values during convalescence. Correlations were found between the fold-change of HCoV-OC43 IgG-S OD values with the fold-change of SARS-CoV-2 IgG-S OD values and C-reactive protein, lactate dehydrogenase, and lymphocyte levels.
Conclusions: There was no association between the antibody titer of seasonal HCoVs in the early phase of COVID-19 and disease severity.
Keywords: COVID-19; Japan; SARS-CoV-2; antibody; cross-reactivity; seasonal human coronavirus.
. 2021 Aug 15;S1201-9712(21)00659-7.
doi: 10.1016/j.ijid.2021.08.026. Online ahead of print.
Cross-reactive humoral immune responses against seasonal human coronaviruses in COVID-19 patients with different disease severity
Kazuo Imai[SUP] 1 [/SUP], Masaru Matsuoka[SUP] 2 [/SUP], Sakiko Tabata[SUP] 3 [/SUP], Yutaro Kitagawa[SUP] 2 [/SUP], Mayu Nagura-Ikeda[SUP] 3 [/SUP], Katsumi Kubota[SUP] 2 [/SUP], Ai Fukada[SUP] 2 [/SUP], Tomohito Takada[SUP] 2 [/SUP], Momoko Sato[SUP] 2 [/SUP], Sakiko Noguchi[SUP] 2 [/SUP], Shinichi Takeuchi[SUP] 2 [/SUP], Noriaki Arakawa[SUP] 4 [/SUP], Kazuyasu Miyoshi[SUP] 3 [/SUP], Yoshiro Saito[SUP] 4 [/SUP], Takuya Maeda[SUP] 2 [/SUP]
Affiliations
- PMID: 34407480
- DOI: 10.1016/j.ijid.2021.08.026
Abstract
Background: We evaluated the cross-reactive antibody response against seasonal human coronaviruses (HCoVs) according to disease severity in patients with COVID-19 in Japan.
Methods: We analyzed 194 paired-collected serum samples from 97 patients with COVID-19 (mild, 35; severe, 62) on admission and during convalescence. IgG antibodies against the nucleocapsid (N) and spike (S) proteins of SARS-CoV-2 and four seasonal HCoVs (HCoV-NL63, -229E, -OC43, and -HKU1) were detected by enzyme-linked immunosorbent assays.
Results: There was no difference in optical density (OD) values for seasonal HCoVs on admission between the severe and mild cases. In addition, a specific pattern of disease severity-associated OD values for HCoVs was not identified. A significant increase of OD values from admission to convalescence for HCoV-HKU1and -OC43 IgG-S and HCoV-NL63 and -229E IgG-N was observed in the severe cases. Significant differences were observed between the mild and severe cases for HCoV-HKU1 and -OC43 IgG-S OD values during convalescence. Correlations were found between the fold-change of HCoV-OC43 IgG-S OD values with the fold-change of SARS-CoV-2 IgG-S OD values and C-reactive protein, lactate dehydrogenase, and lymphocyte levels.
Conclusions: There was no association between the antibody titer of seasonal HCoVs in the early phase of COVID-19 and disease severity.
Keywords: COVID-19; Japan; SARS-CoV-2; antibody; cross-reactivity; seasonal human coronavirus.