tetano
Editor, Senior Moderator
Int J Biol Sci
. 2022 Sep 1;18(15):5591-5606.
doi: 10.7150/ijbs.78002. eCollection 2022.
Serological responses to human virome define clinical outcomes of Italian patients infected with SARS-CoV-2
Limin Wang[SUP] 1 2 [/SUP], Julián Candia[SUP] 1 2 [/SUP], Lichun Ma[SUP] 1 2 [/SUP], Yongmei Zhao[SUP] 3 2 [/SUP], Luisa Imberti[SUP] 4 [/SUP], Alessandra Sottini[SUP] 4 [/SUP], Eugenia Quiros-Roldan[SUP] 5 [/SUP], Kerry Dobbs[SUP] 6 [/SUP], Peter D Burbelo[SUP] 7 [/SUP], Jeffrey I Cohen[SUP] 8 [/SUP], Ottavia M Delmonte[SUP] 6 [/SUP], Marshonna Forgues[SUP] 1 [/SUP], Hui Liu[SUP] 6 [/SUP], Helen F Matthews[SUP] 9 [/SUP], Elana Shaw[SUP] 6 [/SUP], Michael A Stack[SUP] 6 [/SUP], Sarah E Weber[SUP] 10 [/SUP], Yu Zhang[SUP] 6 [/SUP], Andrea Lisco[SUP] 11 [/SUP], Irini Sereti[SUP] 11 [/SUP], Helen C Su[SUP] 6 [/SUP], Luigi D Notarangelo[SUP] 6 [/SUP], Xin Wei Wang[SUP] 1 12 13 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the pandemic respiratory infectious disease COVID-19. However, clinical manifestations and outcomes differ significantly among COVID-19 patients, ranging from asymptomatic to extremely severe, and it remains unclear what drives these disparities. Here, we studied 159 sequentially enrolled hospitalized patients with COVID-19-associated pneumonia from Brescia, Italy using the VirScan phage-display method to characterize circulating antibodies binding to 96,179 viral peptides encoded by 1,276 strains of human viruses. SARS-CoV-2 infection was associated with a marked increase in immune antibody repertoires against many known pathogenic and non-pathogenic human viruses. This antiviral antibody response was linked to longitudinal trajectories of disease severity and was further confirmed in additional 125 COVID-19 patients from the same geographical region in Northern Italy. By applying a machine-learning-based strategy, a viral exposure signature predictive of COVID-19-related disease severity linked to patient survival was developed and validated. These results provide a basis for understanding the role of memory B-cell repertoire to viral epitopes in COVID-19-related symptoms and suggest that a unique anti-viral antibody repertoire signature may be useful to define COVID-19 clinical severity.
. 2022 Sep 1;18(15):5591-5606.
doi: 10.7150/ijbs.78002. eCollection 2022.
Serological responses to human virome define clinical outcomes of Italian patients infected with SARS-CoV-2
Limin Wang[SUP] 1 2 [/SUP], Julián Candia[SUP] 1 2 [/SUP], Lichun Ma[SUP] 1 2 [/SUP], Yongmei Zhao[SUP] 3 2 [/SUP], Luisa Imberti[SUP] 4 [/SUP], Alessandra Sottini[SUP] 4 [/SUP], Eugenia Quiros-Roldan[SUP] 5 [/SUP], Kerry Dobbs[SUP] 6 [/SUP], Peter D Burbelo[SUP] 7 [/SUP], Jeffrey I Cohen[SUP] 8 [/SUP], Ottavia M Delmonte[SUP] 6 [/SUP], Marshonna Forgues[SUP] 1 [/SUP], Hui Liu[SUP] 6 [/SUP], Helen F Matthews[SUP] 9 [/SUP], Elana Shaw[SUP] 6 [/SUP], Michael A Stack[SUP] 6 [/SUP], Sarah E Weber[SUP] 10 [/SUP], Yu Zhang[SUP] 6 [/SUP], Andrea Lisco[SUP] 11 [/SUP], Irini Sereti[SUP] 11 [/SUP], Helen C Su[SUP] 6 [/SUP], Luigi D Notarangelo[SUP] 6 [/SUP], Xin Wei Wang[SUP] 1 12 13 [/SUP]
Affiliations
- PMID: 36263161
- PMCID: PMC9576512
- DOI: 10.7150/ijbs.78002
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the pandemic respiratory infectious disease COVID-19. However, clinical manifestations and outcomes differ significantly among COVID-19 patients, ranging from asymptomatic to extremely severe, and it remains unclear what drives these disparities. Here, we studied 159 sequentially enrolled hospitalized patients with COVID-19-associated pneumonia from Brescia, Italy using the VirScan phage-display method to characterize circulating antibodies binding to 96,179 viral peptides encoded by 1,276 strains of human viruses. SARS-CoV-2 infection was associated with a marked increase in immune antibody repertoires against many known pathogenic and non-pathogenic human viruses. This antiviral antibody response was linked to longitudinal trajectories of disease severity and was further confirmed in additional 125 COVID-19 patients from the same geographical region in Northern Italy. By applying a machine-learning-based strategy, a viral exposure signature predictive of COVID-19-related disease severity linked to patient survival was developed and validated. These results provide a basis for understanding the role of memory B-cell repertoire to viral epitopes in COVID-19-related symptoms and suggest that a unique anti-viral antibody repertoire signature may be useful to define COVID-19 clinical severity.