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Int J Biol Macromol . A molecular docking study revealed that synthetic peptides induced conformational changes in the structure of SARS-CoV-2 spik

tetano

Editor, Senior Moderator
Int J Biol Macromol


. 2020 Jul 18;S0141-8130(20)33937-4.
doi: 10.1016/j.ijbiomac.2020.07.174. Online ahead of print.
A molecular docking study revealed that synthetic peptides induced conformational changes in the structure of SARS-CoV-2 spike glycoprotein, disrupting the interaction with human ACE2 receptor


Pedro F N Souza[SUP] 1 [/SUP], Francisco E S Lopes[SUP] 2 [/SUP], Jackson L Amaral[SUP] 2 [/SUP], Cleverson D T Freitas[SUP] 2 [/SUP], Jose T A Oliveira[SUP] 2 [/SUP]



Affiliations

Abstract

The global outbreak of COVID-19 (Coronavirus Disease 2019) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome caused by Coronavirus 2) began in December 2019. Its closest relative, SARS-CoV-1, has a slightly mutated Spike (S) protein, which interacts with ACE2 receptor in human cells to start the infection. So far, there are no vaccines or drugs to treat COVID-19. So, research groups worldwide are seeking new molecules targeting the S protein to prevent infection by SARS-CoV-2 and COVID-19 establishment. We performed molecular docking analysis of eight synthetic peptides against SARS-CoV-2 S protein. All interacted with the protein, but Mo-CBP[SUB]3[/SUB]-PepII and PepKAA had the highest affinity with it. By binding to the S protein, both peptides led to conformational alterations in the protein, resulting in incorrect interaction with ACE2. Therefore, given the importance of the S protein-ACE2 interaction for SARS-CoV-2 infection, synthetic peptides could block SARS-CoV-2 infection. Moreover, unlike other antiviral drugs, peptides have no toxicity to human cells. Thus, these peptides are potential molecules to be tested against SARS-CoV-2 and to develop new drugs to treat COVID-19.

Keywords: ACE2 receptor; COVID-19; Molecular docking; SARS-CoV-2; Synthetic peptides.
 
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