tetano
Editor, Senior Moderator
Innovation (Camb)
. 2022 Dec 5;100359.
doi: 10.1016/j.xinn.2022.100359. Online ahead of print.
Single-cell transcriptome sequencing reveals the immune response and homeostasis mechanism following administration of BBIBP-CorV SARS-CoV-2 inactivated vaccine
Jianhua Yin[SUP] 1 [/SUP], Yingze Zhao[SUP] 2 3 4 [/SUP], Fubaoqian Huang[SUP] 1 5 [/SUP], Yunkai Yang[SUP] 6 [/SUP], Yaling Huang[SUP] 1 [/SUP], Zhenkun Zhuang[SUP] 1 5 [/SUP], Yanxia Wang[SUP] 7 [/SUP], Zhifeng Wang[SUP] 1 8 [/SUP], Xiumei Lin[SUP] 1 9 [/SUP], Yuhui Zheng[SUP] 1 9 [/SUP], Wenwen Zhou[SUP] 1 10 [/SUP], Shuo Wang[SUP] 1 [/SUP], Ziqian Xu[SUP] 2 [/SUP], Beiwei Ye[SUP] 2 [/SUP], Yaxin Guo[SUP] 2 [/SUP], Wenwen Lei[SUP] 2 [/SUP], Lei Li[SUP] 2 11 [/SUP], Jinmin Tian[SUP] 2 12 [/SUP], Jinxian Gan[SUP] 2 13 [/SUP], Hui Wang[SUP] 14 [/SUP], Wei Wang[SUP] 14 [/SUP], Peiyao Ma[SUP] 1 5 [/SUP], Chang Liu[SUP] 1 [/SUP], Xiaoyu Wei[SUP] 1 15 [/SUP], Xuyang Shi[SUP] 1 15 [/SUP], Zifei Wang[SUP] 1 [/SUP], Yang Wang[SUP] 1 [/SUP], Ying Liu[SUP] 1 9 [/SUP], Mingming Yang[SUP] 16 [/SUP], Yue Yuan[SUP] 1 15 [/SUP], Yumo Song[SUP] 1 [/SUP], Wen Ma[SUP] 1 [/SUP], Zhuoli Huang[SUP] 1 9 [/SUP], Ya Liu[SUP] 1 [/SUP], Yunting Huang[SUP] 17 [/SUP], Haorong Lu[SUP] 17 [/SUP], Peipei Liu[SUP] 2 [/SUP], Hao Liang[SUP] 13 [/SUP], Yong Hou[SUP] 1 8 9 [/SUP], Xun Xu[SUP] 1 18 [/SUP], Longqi Liu[SUP] 1 15 [/SUP], Yuntao Zhang[SUP] 6 [/SUP], Guizhen Wu[SUP] 2 4 [/SUP], George F Gao[SUP] 2 3 4 19 [/SUP], Xin Jin[SUP] 1 20 21 [/SUP], Chuanyu Liu[SUP] 1 15 [/SUP], Xiaoming Yang[SUP] 6 22 [/SUP], William J Liu[SUP] 2 3 4 [/SUP]
Affiliations
Abstract
The BBIBP-CorV severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inactivated vaccine has been authorized for emergency use and widely distributed. We used single-cell transcriptome sequencing to characterize the dynamics of immune responses to the BBIBP-CorV inactivated vaccine. In addition to the expected induction of humoral immunity, we found that the inactivated vaccine induced multiple, comprehensive immune responses, including significantly increased proportions of CD16[SUP]+[/SUP] monocytes and activation of monocyte antigen presentation pathways; T-cell activation pathway upregulation in CD8[SUP]+[/SUP] T cells, along with increased activation of CD4[SUP]+[/SUP] T cells; significant enhancement of cell-cell communications between innate and adaptive immunity; and the induction of regulatory CD4[SUP]+[/SUP] T cells and co-inhibitory interactions to maintain immune homeostasis after vaccination. Additionally, comparative analysis revealed higher neutralizing antibody levels, distinct expansion of naïve T cells, a shared increased proportion of regulatory CD4[SUP]+[/SUP] T cells, and upregulated expression of functional genes in booster dose recipients with a longer interval after the second vaccination. Our research will support a comprehensive understanding of the systemic immune responses elicited by the BBIBP-CorV inactivated vaccine, which will facilitate the formulation of better vaccination strategies and the design of new vaccines.
. 2022 Dec 5;100359.
doi: 10.1016/j.xinn.2022.100359. Online ahead of print.
Single-cell transcriptome sequencing reveals the immune response and homeostasis mechanism following administration of BBIBP-CorV SARS-CoV-2 inactivated vaccine
Jianhua Yin[SUP] 1 [/SUP], Yingze Zhao[SUP] 2 3 4 [/SUP], Fubaoqian Huang[SUP] 1 5 [/SUP], Yunkai Yang[SUP] 6 [/SUP], Yaling Huang[SUP] 1 [/SUP], Zhenkun Zhuang[SUP] 1 5 [/SUP], Yanxia Wang[SUP] 7 [/SUP], Zhifeng Wang[SUP] 1 8 [/SUP], Xiumei Lin[SUP] 1 9 [/SUP], Yuhui Zheng[SUP] 1 9 [/SUP], Wenwen Zhou[SUP] 1 10 [/SUP], Shuo Wang[SUP] 1 [/SUP], Ziqian Xu[SUP] 2 [/SUP], Beiwei Ye[SUP] 2 [/SUP], Yaxin Guo[SUP] 2 [/SUP], Wenwen Lei[SUP] 2 [/SUP], Lei Li[SUP] 2 11 [/SUP], Jinmin Tian[SUP] 2 12 [/SUP], Jinxian Gan[SUP] 2 13 [/SUP], Hui Wang[SUP] 14 [/SUP], Wei Wang[SUP] 14 [/SUP], Peiyao Ma[SUP] 1 5 [/SUP], Chang Liu[SUP] 1 [/SUP], Xiaoyu Wei[SUP] 1 15 [/SUP], Xuyang Shi[SUP] 1 15 [/SUP], Zifei Wang[SUP] 1 [/SUP], Yang Wang[SUP] 1 [/SUP], Ying Liu[SUP] 1 9 [/SUP], Mingming Yang[SUP] 16 [/SUP], Yue Yuan[SUP] 1 15 [/SUP], Yumo Song[SUP] 1 [/SUP], Wen Ma[SUP] 1 [/SUP], Zhuoli Huang[SUP] 1 9 [/SUP], Ya Liu[SUP] 1 [/SUP], Yunting Huang[SUP] 17 [/SUP], Haorong Lu[SUP] 17 [/SUP], Peipei Liu[SUP] 2 [/SUP], Hao Liang[SUP] 13 [/SUP], Yong Hou[SUP] 1 8 9 [/SUP], Xun Xu[SUP] 1 18 [/SUP], Longqi Liu[SUP] 1 15 [/SUP], Yuntao Zhang[SUP] 6 [/SUP], Guizhen Wu[SUP] 2 4 [/SUP], George F Gao[SUP] 2 3 4 19 [/SUP], Xin Jin[SUP] 1 20 21 [/SUP], Chuanyu Liu[SUP] 1 15 [/SUP], Xiaoming Yang[SUP] 6 22 [/SUP], William J Liu[SUP] 2 3 4 [/SUP]
Affiliations
- PMID: 36506806
- PMCID: PMC9719934
- DOI: 10.1016/j.xinn.2022.100359
Abstract
The BBIBP-CorV severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inactivated vaccine has been authorized for emergency use and widely distributed. We used single-cell transcriptome sequencing to characterize the dynamics of immune responses to the BBIBP-CorV inactivated vaccine. In addition to the expected induction of humoral immunity, we found that the inactivated vaccine induced multiple, comprehensive immune responses, including significantly increased proportions of CD16[SUP]+[/SUP] monocytes and activation of monocyte antigen presentation pathways; T-cell activation pathway upregulation in CD8[SUP]+[/SUP] T cells, along with increased activation of CD4[SUP]+[/SUP] T cells; significant enhancement of cell-cell communications between innate and adaptive immunity; and the induction of regulatory CD4[SUP]+[/SUP] T cells and co-inhibitory interactions to maintain immune homeostasis after vaccination. Additionally, comparative analysis revealed higher neutralizing antibody levels, distinct expansion of naïve T cells, a shared increased proportion of regulatory CD4[SUP]+[/SUP] T cells, and upregulated expression of functional genes in booster dose recipients with a longer interval after the second vaccination. Our research will support a comprehensive understanding of the systemic immune responses elicited by the BBIBP-CorV inactivated vaccine, which will facilitate the formulation of better vaccination strategies and the design of new vaccines.