tetano
Editor, Senior Moderator
J Virol Methods. 2011 Sep 17. [Epub ahead of print]
Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro.
Xiang DX, Chen Q, Pang L, Zheng CL.
Source
Department of Microbiology, Medical College, Dalian University, Dalian 116622, Liaoning, China.
Abstract
Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus.
Copyright ? 2011. Published by Elsevier B.V.
PMID:
21945220
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21945220
Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro.
Xiang DX, Chen Q, Pang L, Zheng CL.
Source
Department of Microbiology, Medical College, Dalian University, Dalian 116622, Liaoning, China.
Abstract
Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus.
Copyright ? 2011. Published by Elsevier B.V.
PMID:
21945220
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21945220