tetano
Editor, Senior Moderator
Sheng Wu Gong Cheng Xue Bao. 2019 Jun 25;35(6):1029-1040. doi: 10.13345/j.cjb.180490.
[h=1][Inhibition of the replication of H9N2 influenza virus in vivo by short-term repeated oral administration of chicken interferon α].[/h] [Article in Chinese; Abstract available in Chinese from the publisher]
Wang M[SUP]1,[/SUP][SUP]2[/SUP], Song J[SUP]1,[/SUP][SUP]2[/SUP], Fan W[SUP]1[/SUP], Liu L[SUP]1,[/SUP][SUP]2[/SUP], Huang Z[SUP]3[/SUP], Yang C[SUP]3[/SUP], Wu H[SUP]3[/SUP], Liu W[SUP]1,[/SUP][SUP]2[/SUP], Li J[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] in English, Chinese
To evaluate the optimal administration frequency for interferon-α (IFN-α) and the effect of its combined use with inactive virus on chicken flocks, the prokaryotic expression plasmid pET-22b-ChIFN-α was constructed and transferred into Escherichia coli BL21(DE3) host bacteria to induce the expression of chicken IFN-α and to harvest recombinant proteins inclusion bodies. The expression of recombinant chicken IFN-α was confirmed by SDS-PAGE, and the results demonstrated that the chicken IFN-α (20 kDa) was highly expressed using the prokaryotic expression vector with a concentration of 0.2 mg/mL in the medium. Chicken IFN-α was diluted to 2.5?10⁴ U/fowls and administered to immunized specific-pathogen-free chickens orally in combination with inactivated H9N2 subtype influenza virus. Chicken that received chicken IFN-α were safe after three repeated immunizations (96 h). In addition, chicken IFN-α could induce higher levels of antiviral-related inducible genes in peripheral blood, spleen, and thymus of chicken flocks. The results of a challenge assay revealed that the lowest detoxification rates of chicken IFN-α ranged from three to five days, suggesting a higher capacity to resist H9N2 subtype avian influenza virus. The present study obtained the optimal immune frequency and immunization period for chicken IFN-α to provide theoretical support for the optimal clinical application of IFN-α.
[h=4]KEYWORDS:[/h] H9N2 influenza viruses; chicken interferon α; oral administration; short-term repeated
PMID: 31231999 DOI: 10.13345/j.cjb.180490
[h=1][Inhibition of the replication of H9N2 influenza virus in vivo by short-term repeated oral administration of chicken interferon α].[/h] [Article in Chinese; Abstract available in Chinese from the publisher]
Wang M[SUP]1,[/SUP][SUP]2[/SUP], Song J[SUP]1,[/SUP][SUP]2[/SUP], Fan W[SUP]1[/SUP], Liu L[SUP]1,[/SUP][SUP]2[/SUP], Huang Z[SUP]3[/SUP], Yang C[SUP]3[/SUP], Wu H[SUP]3[/SUP], Liu W[SUP]1,[/SUP][SUP]2[/SUP], Li J[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] in English, Chinese
To evaluate the optimal administration frequency for interferon-α (IFN-α) and the effect of its combined use with inactive virus on chicken flocks, the prokaryotic expression plasmid pET-22b-ChIFN-α was constructed and transferred into Escherichia coli BL21(DE3) host bacteria to induce the expression of chicken IFN-α and to harvest recombinant proteins inclusion bodies. The expression of recombinant chicken IFN-α was confirmed by SDS-PAGE, and the results demonstrated that the chicken IFN-α (20 kDa) was highly expressed using the prokaryotic expression vector with a concentration of 0.2 mg/mL in the medium. Chicken IFN-α was diluted to 2.5?10⁴ U/fowls and administered to immunized specific-pathogen-free chickens orally in combination with inactivated H9N2 subtype influenza virus. Chicken that received chicken IFN-α were safe after three repeated immunizations (96 h). In addition, chicken IFN-α could induce higher levels of antiviral-related inducible genes in peripheral blood, spleen, and thymus of chicken flocks. The results of a challenge assay revealed that the lowest detoxification rates of chicken IFN-α ranged from three to five days, suggesting a higher capacity to resist H9N2 subtype avian influenza virus. The present study obtained the optimal immune frequency and immunization period for chicken IFN-α to provide theoretical support for the optimal clinical application of IFN-α.
[h=4]KEYWORDS:[/h] H9N2 influenza viruses; chicken interferon α; oral administration; short-term repeated
PMID: 31231999 DOI: 10.13345/j.cjb.180490