tetano
Editor, Senior Moderator
Virol J. 2019 Dec 23;16(1):163. doi: 10.1186/s12985-019-1266-x. [h=1]Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways.[/h]
Yu Y[SUP]1[/SUP], Zhang Y[SUP]1[/SUP], Wang S[SUP]1[/SUP], Liu W[SUP]1[/SUP], Hao C[SUP]2[/SUP], Wang W[SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao, 266003, People's Republic of China. 2 Systems Biology & Medicine Center for Complex Diseases, Affiliated Hospital of Qingdao University, Qingdao, 266003, People's Republic of China. haocui@qduhospital.cn. 3 Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao, 266003, People's Republic of China. wwwakin@ouc.edu.cn. 4 Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, People's Republic of China. wwwakin@ouc.edu.cn.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects.
[h=4]METHODS:[/h] In this study, the anti-influenza virus (IAV) activities and mechanisms were investigated both in vitro and in vivo. The inhibitory effects of PA against IAV in vitro were evaluated by plaque assay and immunofluorescence assay. The neuraminidase inhibition assay, hemagglutination inhibition (HI) assay, and western blot assay were used to explore the anti-viral mechanisms. The anti-IAV activities in vivo were determined by mice pneumonia model and HE staining.
[h=4]RESULTS:[/h] The results showed that PA significantly inhibited different IAV strains multiplication in vitro, and may block IAV infection through inactivating virus particles directly and interfering with some early stages after virus adsorption. Cellular PI3K/Akt and ERK/MAPK signaling pathways may be involved in the anti-IAV actions of PA. Intranasal administration of PA markedly improved mice survival and attenuated pneumonia symptoms in IAV infected mice, comparable to the effects of Oseltamivir.
[h=4]CONCLUSIONS:[/h] Therefore, Patchouli alcohol has the potential to be developed into a novel anti-IAV agent in the future.
[h=4]KEYWORDS:[/h] Antiviral effects; ERK/MAPK pathway; Influenza a virus; PI3K/Akt pathway; Patchouli alcohol
PMID: 31870450 DOI: 10.1186/s12985-019-1266-x
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Yu Y[SUP]1[/SUP], Zhang Y[SUP]1[/SUP], Wang S[SUP]1[/SUP], Liu W[SUP]1[/SUP], Hao C[SUP]2[/SUP], Wang W[SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao, 266003, People's Republic of China. 2 Systems Biology & Medicine Center for Complex Diseases, Affiliated Hospital of Qingdao University, Qingdao, 266003, People's Republic of China. haocui@qduhospital.cn. 3 Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao, 266003, People's Republic of China. wwwakin@ouc.edu.cn. 4 Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, People's Republic of China. wwwakin@ouc.edu.cn.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects.
[h=4]METHODS:[/h] In this study, the anti-influenza virus (IAV) activities and mechanisms were investigated both in vitro and in vivo. The inhibitory effects of PA against IAV in vitro were evaluated by plaque assay and immunofluorescence assay. The neuraminidase inhibition assay, hemagglutination inhibition (HI) assay, and western blot assay were used to explore the anti-viral mechanisms. The anti-IAV activities in vivo were determined by mice pneumonia model and HE staining.
[h=4]RESULTS:[/h] The results showed that PA significantly inhibited different IAV strains multiplication in vitro, and may block IAV infection through inactivating virus particles directly and interfering with some early stages after virus adsorption. Cellular PI3K/Akt and ERK/MAPK signaling pathways may be involved in the anti-IAV actions of PA. Intranasal administration of PA markedly improved mice survival and attenuated pneumonia symptoms in IAV infected mice, comparable to the effects of Oseltamivir.
[h=4]CONCLUSIONS:[/h] Therefore, Patchouli alcohol has the potential to be developed into a novel anti-IAV agent in the future.
[h=4]KEYWORDS:[/h] Antiviral effects; ERK/MAPK pathway; Influenza a virus; PI3K/Akt pathway; Patchouli alcohol
PMID: 31870450 DOI: 10.1186/s12985-019-1266-x
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