tetano
Editor, Senior Moderator
Anal Chem. 2012 Sep 14. [Epub ahead of print]
Influenza Virus-Membrane Fusion Triggered by Rapid Proton Uncaging for Single Particle Studies of Fusion Kinetics.
Costello DA, Lee DW, Drewes J, Vasquez KA, Kisler K, Wiesner U, Pollack L, Whittaker GR, Daniel S.
Abstract
We report a method for studying membrane fusion, focusing on influenza virus fusion to lipid bilayers, which provides high temporal resolution through the rapid and coordinated initiation of individual virus fusion events. Each fusion event proceeds through a series of steps, much like multi-step chemical reaction. Fusion is initiated by a rapid decrease in pH that accompanies the 'uncaging' of an effector molecule from o-nitrobenzaldehyde, a photoisomerizable compound that releases a proton to the surrounding solution within microseconds of long-wave ultraviolet irradiation. In order to quantify pH values upon UV irradiation and uncaging, we introduce a simple silica nanoparticle pH sensor, useful for reporting the pH in homogeneous nanoliter volumes under conditions where traditional organic dye-type pH probes fail. Subsequent single-virion fusion events are monitored using total internal reflection fluorescence microscopy. Statistical analysis of these stochastic events uncovers kinetic information about the fusion reaction. This approach reveals that the kinetic parameters obtained from the data are sensitive to the rate at which protons are delivered to the bound viruses. Higher resolution measurements can enhance fundamental fusion studies and aid anti-viral anti-fusogenic drug development.
PMID:
22974237
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22974237
Influenza Virus-Membrane Fusion Triggered by Rapid Proton Uncaging for Single Particle Studies of Fusion Kinetics.
Costello DA, Lee DW, Drewes J, Vasquez KA, Kisler K, Wiesner U, Pollack L, Whittaker GR, Daniel S.
Abstract
We report a method for studying membrane fusion, focusing on influenza virus fusion to lipid bilayers, which provides high temporal resolution through the rapid and coordinated initiation of individual virus fusion events. Each fusion event proceeds through a series of steps, much like multi-step chemical reaction. Fusion is initiated by a rapid decrease in pH that accompanies the 'uncaging' of an effector molecule from o-nitrobenzaldehyde, a photoisomerizable compound that releases a proton to the surrounding solution within microseconds of long-wave ultraviolet irradiation. In order to quantify pH values upon UV irradiation and uncaging, we introduce a simple silica nanoparticle pH sensor, useful for reporting the pH in homogeneous nanoliter volumes under conditions where traditional organic dye-type pH probes fail. Subsequent single-virion fusion events are monitored using total internal reflection fluorescence microscopy. Statistical analysis of these stochastic events uncovers kinetic information about the fusion reaction. This approach reveals that the kinetic parameters obtained from the data are sensitive to the rate at which protons are delivered to the bound viruses. Higher resolution measurements can enhance fundamental fusion studies and aid anti-viral anti-fusogenic drug development.
PMID:
22974237
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22974237