tetano
Editor, Senior Moderator
Clin Immunol. 2014 Aug 13. pii: S1521-6616(14)00194-6. doi: 10.1016/j.clim.2014.08.003. [Epub ahead of print]
Influenza Virus-Like Particle Vaccines made in Nicotiana benthamiana Elicit Durable, Poly-Functional and Cross-Reactive T Cell Responses to Influenza HA Antigens.
Landry N1, Pillet S2, Favre D3, Poulin JF4, Tr?panier S5, Yassine-Diab B6, Ward BJ7.
Author information
Abstract
Cell-mediated immunity plays a major role in long-lived, cross-reactive protection against influenza virus. We measured long-term poly-functional and cross-reactive T cell responses to influenza hemagglutinin (HA) elicited by a new plant-made Virus-Like Particle (VLP) vaccine targeting either H1N1 A/California/7/09 (H1) or H5N1 A/Indonesia/5/05 (H5). In two independent clinical trials, we characterized the CD4+ and CD8+ T cell homotypic and heterotypic responses 6months after different vaccination regimens. Responses of VLP-vaccinated subjects were compared with placebo and/or a commercial trivalent inactivated vaccine (TIV:Fluzone?) recipients. Both H1 and H5 VLP vaccines elicited significantly greater poly-functional CD4+ T cell responses than placebo and TIV. Poly-functional CD8+ T cell responses were also observed after H1 VLP vaccination. Our results show that plant-made HA VLP vaccines elicit both strong antibody responses and poly-functional, cross-reactive memory T cells that persist for at least 6months after vaccination.
Copyright ? 2014. Published by Elsevier Inc.
KEYWORDS:
Influenza; Nicotiana benthamiana; Vaccine; Virus-like particle; cell-mediated immunity; long-term memory response
PMID:
25128897
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25128897
Influenza Virus-Like Particle Vaccines made in Nicotiana benthamiana Elicit Durable, Poly-Functional and Cross-Reactive T Cell Responses to Influenza HA Antigens.
Landry N1, Pillet S2, Favre D3, Poulin JF4, Tr?panier S5, Yassine-Diab B6, Ward BJ7.
Author information
Abstract
Cell-mediated immunity plays a major role in long-lived, cross-reactive protection against influenza virus. We measured long-term poly-functional and cross-reactive T cell responses to influenza hemagglutinin (HA) elicited by a new plant-made Virus-Like Particle (VLP) vaccine targeting either H1N1 A/California/7/09 (H1) or H5N1 A/Indonesia/5/05 (H5). In two independent clinical trials, we characterized the CD4+ and CD8+ T cell homotypic and heterotypic responses 6months after different vaccination regimens. Responses of VLP-vaccinated subjects were compared with placebo and/or a commercial trivalent inactivated vaccine (TIV:Fluzone?) recipients. Both H1 and H5 VLP vaccines elicited significantly greater poly-functional CD4+ T cell responses than placebo and TIV. Poly-functional CD8+ T cell responses were also observed after H1 VLP vaccination. Our results show that plant-made HA VLP vaccines elicit both strong antibody responses and poly-functional, cross-reactive memory T cells that persist for at least 6months after vaccination.
Copyright ? 2014. Published by Elsevier Inc.
KEYWORDS:
Influenza; Nicotiana benthamiana; Vaccine; Virus-like particle; cell-mediated immunity; long-term memory response
PMID:
25128897
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25128897