tetano
Editor, Senior Moderator
Aging Cell. 2019 Jan 11:e12893. doi: 10.1111/acel.12893. [Epub ahead of print]
[h=1]Influenza virus inoculum volume is critical to elucidate age-dependent mortality in mice.[/h] Smith CA[SUP]1[/SUP], Kulkarni U[SUP]1[/SUP], Chen J[SUP]2[/SUP], Goldstein DR[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The elderly exhibit increased mortality to influenza viral infection for unclear reasons. Mice are frequently used to model how aging impacts disease. Several studies have shown that aged mice exhibit an increased mortality to influenza virus, but two recent studies demonstrated the opposite. These two studies administered the virus intranasally in 20 ?L, whereas the other studies used a viral inoculum in at least 30 ?L. To determine whether the volume of the inoculum could explain the conflicting reports, we infected young and aged mice via intranasal instillation of 40 ?L or 20 ?L containing 1 x 10[SUP]4[/SUP] plaque-forming units (PFU) of H1N1 influenza virus. We found that intranasal administration of 40 ?L but not 20 ?L of inoculum resulted in age-dependent mortality in mice. Compared to aged mice infected with 40 ?L inoculum, those infected with 20 ?L inoculum showed reduced levels of live virus and IFN-β in the lung 3 days postinfection. Furthermore, aged mice administered 40 ?L of Evans blue intranasally displayed increased dye retention in their bronchoalveolar lavage fluid compared to those administered 20 ?L of Evans blue. Our data demonstrate that the inoculating volume of virus is critical for adequate delivery of influenza virus to the lung and thus for efficient infection of aged mice. These findings shed light on discrepant results in the literature regarding aged mice and influenza infection, and establish that mice can be used to examine how aging impacts the response to this biomedically important infection.
? 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
PMID: 30632262 DOI: 10.1111/acel.12893
Free full text
[h=1]Influenza virus inoculum volume is critical to elucidate age-dependent mortality in mice.[/h] Smith CA[SUP]1[/SUP], Kulkarni U[SUP]1[/SUP], Chen J[SUP]2[/SUP], Goldstein DR[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The elderly exhibit increased mortality to influenza viral infection for unclear reasons. Mice are frequently used to model how aging impacts disease. Several studies have shown that aged mice exhibit an increased mortality to influenza virus, but two recent studies demonstrated the opposite. These two studies administered the virus intranasally in 20 ?L, whereas the other studies used a viral inoculum in at least 30 ?L. To determine whether the volume of the inoculum could explain the conflicting reports, we infected young and aged mice via intranasal instillation of 40 ?L or 20 ?L containing 1 x 10[SUP]4[/SUP] plaque-forming units (PFU) of H1N1 influenza virus. We found that intranasal administration of 40 ?L but not 20 ?L of inoculum resulted in age-dependent mortality in mice. Compared to aged mice infected with 40 ?L inoculum, those infected with 20 ?L inoculum showed reduced levels of live virus and IFN-β in the lung 3 days postinfection. Furthermore, aged mice administered 40 ?L of Evans blue intranasally displayed increased dye retention in their bronchoalveolar lavage fluid compared to those administered 20 ?L of Evans blue. Our data demonstrate that the inoculating volume of virus is critical for adequate delivery of influenza virus to the lung and thus for efficient infection of aged mice. These findings shed light on discrepant results in the literature regarding aged mice and influenza infection, and establish that mice can be used to examine how aging impacts the response to this biomedically important infection.
? 2018 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
PMID: 30632262 DOI: 10.1111/acel.12893
Free full text