tetano
Editor, Senior Moderator
Sci Rep. 2017 Jan 6;7:40360. doi: 10.1038/srep40360.
[h=1]Influenza virus exploits tunneling nanotubes for cell-to-cell spread.[/h] Kumar A[SUP]1[/SUP], Kim JH[SUP]1[/SUP], Ranjan P[SUP]1[/SUP], Metcalfe MG[SUP]2[/SUP], Cao W[SUP]1[/SUP], Mishina M[SUP]1[/SUP], Gangappa S[SUP]1[/SUP], Guo Z[SUP]3[/SUP], Boyden ES[SUP]4[/SUP], Zaki S[SUP]2[/SUP], York I[SUP]1[/SUP], Garc?a-Sastre A[SUP]5[/SUP], Shaw M[SUP]6[/SUP], Sambhara S[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Tunneling nanotubes (TNTs) represent a novel route of intercellular communication. While previous work has shown that TNTs facilitate the exchange of viral or prion proteins from infected to na?ve cells, it is not clear whether the viral genome is also transferred via this mechanism and further, whether transfer via this route can result in productive replication of the infectious agents in the recipient cell. Here we present evidence that lung epithelial cells are connected by TNTs, and in spite of the presence of neutralizing antibodies and an antiviral agent, Oseltamivir, influenza virus can exploit these networks to transfer viral proteins and genome from the infected to na?ve cell, resulting in productive viral replication in the na?ve cells. These observations indicate that influenza viruses can spread using these intercellular networks that connect epithelial cells, evading immune and antiviral defenses and provide an explanation for the incidence of influenza infections even in influenza-immune individuals and vaccine failures.
PMID: 28059146 DOI: 10.1038/srep40360
[PubMed - in process]
[h=1]Influenza virus exploits tunneling nanotubes for cell-to-cell spread.[/h] Kumar A[SUP]1[/SUP], Kim JH[SUP]1[/SUP], Ranjan P[SUP]1[/SUP], Metcalfe MG[SUP]2[/SUP], Cao W[SUP]1[/SUP], Mishina M[SUP]1[/SUP], Gangappa S[SUP]1[/SUP], Guo Z[SUP]3[/SUP], Boyden ES[SUP]4[/SUP], Zaki S[SUP]2[/SUP], York I[SUP]1[/SUP], Garc?a-Sastre A[SUP]5[/SUP], Shaw M[SUP]6[/SUP], Sambhara S[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Tunneling nanotubes (TNTs) represent a novel route of intercellular communication. While previous work has shown that TNTs facilitate the exchange of viral or prion proteins from infected to na?ve cells, it is not clear whether the viral genome is also transferred via this mechanism and further, whether transfer via this route can result in productive replication of the infectious agents in the recipient cell. Here we present evidence that lung epithelial cells are connected by TNTs, and in spite of the presence of neutralizing antibodies and an antiviral agent, Oseltamivir, influenza virus can exploit these networks to transfer viral proteins and genome from the infected to na?ve cell, resulting in productive viral replication in the na?ve cells. These observations indicate that influenza viruses can spread using these intercellular networks that connect epithelial cells, evading immune and antiviral defenses and provide an explanation for the incidence of influenza infections even in influenza-immune individuals and vaccine failures.
PMID: 28059146 DOI: 10.1038/srep40360
[PubMed - in process]