tetano
Editor, Senior Moderator
J Clin Virol. 2015 Nov 4;73:105-107. doi: 10.1016/j.jcv.2015.11.002. [Epub ahead of print]
[h=1]Influenza virus emitted by naturally-infected hosts in a healthcare setting.[/h] Mubareka S[SUP]1[/SUP], Granados A[SUP]2[/SUP], Naik U[SUP]3[/SUP], Darwish I[SUP]4[/SUP], Cutts TA[SUP]5[/SUP], Astrakianakis G[SUP]6[/SUP], Gubbay JB[SUP]7[/SUP], Peci A[SUP]8[/SUP], Scott JA[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The emergence of novel respiratory viruses such as avian influenza A(H7N9) virus and the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) highlights the importance of understanding determinants of transmission to healthcare workers (HCWs) and the public.
[h=4]OBJECTIVES:[/h] We aim to determine the viral content of the air emitted by symptomatic inpatients or long-term care residents with laboratory-confirmed influenza virus infection (emitters), and in the breathing zones of healthcare workers who attend to them.
[h=4]DESIGN:[/h] A prospective pilot study of patients with laboratory-confirmed influenza virus infection was undertaken. Air within 1m of the patient was sampled using a high volume air sampler. In addition, a lower volume air sampler was placed <1m from the patient, with another >1m from the patient. Viral RNA was recovered from the samplers and submitted for quantitative real time PCR. In addition, personal button samplers were provided to HCWs.
[h=4]RESULTS:[/h] The air emitted by 15 participants with laboratory-confirmed influenza virus infection was sampled. Of the patients infected with influenza A, viral RNA was recovered from the air emitted by 9/12 patients using the low-volume sampler; no viral RNA was detected from air emitted by patients with influenza B (n=3). Influenza virus RNA was recovered from one HCW's sampler.
[h=4]CONCLUSIONS:[/h] Patients with respiratory virus infection emit virus into the air which disperses to >1m and may reach the breathing zone of a HCW. This pilot study highlights the feasibility and importance of conducting a larger-scale study to identify determinants of exposure and transmission from patient to HCW.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Bioaerosol; Exposure; Healthcare; Influenza virus; Transmission
PMID: 26590688 [PubMed - as supplied by publisher]
[h=1]Influenza virus emitted by naturally-infected hosts in a healthcare setting.[/h] Mubareka S[SUP]1[/SUP], Granados A[SUP]2[/SUP], Naik U[SUP]3[/SUP], Darwish I[SUP]4[/SUP], Cutts TA[SUP]5[/SUP], Astrakianakis G[SUP]6[/SUP], Gubbay JB[SUP]7[/SUP], Peci A[SUP]8[/SUP], Scott JA[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] The emergence of novel respiratory viruses such as avian influenza A(H7N9) virus and the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) highlights the importance of understanding determinants of transmission to healthcare workers (HCWs) and the public.
[h=4]OBJECTIVES:[/h] We aim to determine the viral content of the air emitted by symptomatic inpatients or long-term care residents with laboratory-confirmed influenza virus infection (emitters), and in the breathing zones of healthcare workers who attend to them.
[h=4]DESIGN:[/h] A prospective pilot study of patients with laboratory-confirmed influenza virus infection was undertaken. Air within 1m of the patient was sampled using a high volume air sampler. In addition, a lower volume air sampler was placed <1m from the patient, with another >1m from the patient. Viral RNA was recovered from the samplers and submitted for quantitative real time PCR. In addition, personal button samplers were provided to HCWs.
[h=4]RESULTS:[/h] The air emitted by 15 participants with laboratory-confirmed influenza virus infection was sampled. Of the patients infected with influenza A, viral RNA was recovered from the air emitted by 9/12 patients using the low-volume sampler; no viral RNA was detected from air emitted by patients with influenza B (n=3). Influenza virus RNA was recovered from one HCW's sampler.
[h=4]CONCLUSIONS:[/h] Patients with respiratory virus infection emit virus into the air which disperses to >1m and may reach the breathing zone of a HCW. This pilot study highlights the feasibility and importance of conducting a larger-scale study to identify determinants of exposure and transmission from patient to HCW.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Bioaerosol; Exposure; Healthcare; Influenza virus; Transmission
PMID: 26590688 [PubMed - as supplied by publisher]