tetano
Editor, Senior Moderator
J Gen Virol. 2013 Jan 3. [Epub ahead of print]
Influenza virus budding from the tips of cellular microvilli in differentiated human airway epithelial cells.
Kolesnikova L, Heck S, Matrosovich T, Klenk HD, Becker S, Matrosovich M.
Source
Institute of Virology, Philipps University Marburg, Germany.
Abstract
The epithelium of conducting airways represents the main target for influenza virus in mammals, however peculiarities of virus interactions with differentiated airway epithelial cells remain largely unknown. We studied influenza virus budding in differentiated cultures of human tracheo-bronchial epithelial cells by using transmission electron microscopy. Budding of spherical and filamentous virions were observed on the apical surfaces of cells with no association with cilia and secretory granules. Quantitative analysis of the distribution of viral buds on the cell surface indicated that tips of microvilli represent a prominent site of influenza virus budding in the human airway epithelium. Since microvilli of differentiated cells are involved in many fundamental cell functions, our data prompt further studies on the biological significance of microvilli-associated budding for the virus replication, transmission and pathogenicity.
PMID:
23288421
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23288421
Influenza virus budding from the tips of cellular microvilli in differentiated human airway epithelial cells.
Kolesnikova L, Heck S, Matrosovich T, Klenk HD, Becker S, Matrosovich M.
Source
Institute of Virology, Philipps University Marburg, Germany.
Abstract
The epithelium of conducting airways represents the main target for influenza virus in mammals, however peculiarities of virus interactions with differentiated airway epithelial cells remain largely unknown. We studied influenza virus budding in differentiated cultures of human tracheo-bronchial epithelial cells by using transmission electron microscopy. Budding of spherical and filamentous virions were observed on the apical surfaces of cells with no association with cilia and secretory granules. Quantitative analysis of the distribution of viral buds on the cell surface indicated that tips of microvilli represent a prominent site of influenza virus budding in the human airway epithelium. Since microvilli of differentiated cells are involved in many fundamental cell functions, our data prompt further studies on the biological significance of microvilli-associated budding for the virus replication, transmission and pathogenicity.
PMID:
23288421
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23288421