tetano
Editor, Senior Moderator
Influenza Other Respir Viruses
. 2020 Jun 13.
doi: 10.1111/irv.12760. Online ahead of print.
The Antiviral Effects of Baloxavir Marboxil Against Influenza A Virus Infection in Ferrets
Mitsutaka Kitano[SUP] 1 [/SUP], Takanobu Matsuzaki[SUP] 1 [/SUP], Ryoko Oka[SUP] 1 [/SUP], Kaoru Baba[SUP] 2 [/SUP], Takahiro Noda[SUP] 2 [/SUP], Yuki Yoshida[SUP] 1 [/SUP], Kenji Sato[SUP] 1 [/SUP], Kohei Kiyota[SUP] 1 [/SUP], Tohru Mizutare[SUP] 1 [/SUP], Ryu Yoshida[SUP] 1 [/SUP], Akihiko Sato[SUP] 1 [/SUP], Hiroshi Kamimori[SUP] 1 [/SUP], Takao Shishido[SUP] 1 [/SUP], Akira Naito[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Baloxavir marboxil (BXM), the oral prodrug of baloxavir acid (BXA), greatly reduces virus titers as well as influenza symptoms of uncomplicated influenza in patients.
Objectives: To investigate the pharmacokinetic profiles of BXA and its efficacy against influenza A virus infection in ferrets.
Methods: Ferrets were dosed orally with BXM (10 and 30 mg/kg twice daily for 1 day), oseltamivir phosphate (OSP) (5 mg/kg twice daily for 2 days) or vehicle to measure the antiviral effects of BXM and OSP. The pharmacokinetic parameters of BXA was determined after single oral dosing of BXM.
Results: The maximum plasma concentrations of BXA were observed at 1.50 and 2.00 hours with the two BXM doses, which then declined with an elimination half-life of 6.91 and 4.44 hours, respectively. BXM at both doses remained detectable in the plasma in ferrets, which may be due to higher stability in liver microsomes. BXM (10 and 30 mg/kg twice daily) treatment at Day 1 post-infection (p.i.) reduced virus titers by ≥3 log10 of the 50% tissue culture infective doses by Day 2, which was significantly different compared with vehicle or OSP. Body temperature drops over time were significantly greater with BXM than with vehicle or OSP. Significant reduction in virus titers was also demonstrated when BXM was administrated after symptom onset at Day 2 p.i. compared with vehicle and OSP, although body temperature changes largely overlapped between Day 2 and Day 4.
Conclusions: The results highlight the rapid antiviral action of BXM with post-exposure prophylaxis or therapeutic dosing in ferrets and offer support for further research on prevention of influenza virus infection and transmission.
Keywords: baloxavir marboxil; ferrets; influenza A virus; pharmacodynamics; pharmacokinetics.
. 2020 Jun 13.
doi: 10.1111/irv.12760. Online ahead of print.
The Antiviral Effects of Baloxavir Marboxil Against Influenza A Virus Infection in Ferrets
Mitsutaka Kitano[SUP] 1 [/SUP], Takanobu Matsuzaki[SUP] 1 [/SUP], Ryoko Oka[SUP] 1 [/SUP], Kaoru Baba[SUP] 2 [/SUP], Takahiro Noda[SUP] 2 [/SUP], Yuki Yoshida[SUP] 1 [/SUP], Kenji Sato[SUP] 1 [/SUP], Kohei Kiyota[SUP] 1 [/SUP], Tohru Mizutare[SUP] 1 [/SUP], Ryu Yoshida[SUP] 1 [/SUP], Akihiko Sato[SUP] 1 [/SUP], Hiroshi Kamimori[SUP] 1 [/SUP], Takao Shishido[SUP] 1 [/SUP], Akira Naito[SUP] 1 [/SUP]
Affiliations
- PMID: 32533654
- DOI: 10.1111/irv.12760
Abstract
Background: Baloxavir marboxil (BXM), the oral prodrug of baloxavir acid (BXA), greatly reduces virus titers as well as influenza symptoms of uncomplicated influenza in patients.
Objectives: To investigate the pharmacokinetic profiles of BXA and its efficacy against influenza A virus infection in ferrets.
Methods: Ferrets were dosed orally with BXM (10 and 30 mg/kg twice daily for 1 day), oseltamivir phosphate (OSP) (5 mg/kg twice daily for 2 days) or vehicle to measure the antiviral effects of BXM and OSP. The pharmacokinetic parameters of BXA was determined after single oral dosing of BXM.
Results: The maximum plasma concentrations of BXA were observed at 1.50 and 2.00 hours with the two BXM doses, which then declined with an elimination half-life of 6.91 and 4.44 hours, respectively. BXM at both doses remained detectable in the plasma in ferrets, which may be due to higher stability in liver microsomes. BXM (10 and 30 mg/kg twice daily) treatment at Day 1 post-infection (p.i.) reduced virus titers by ≥3 log10 of the 50% tissue culture infective doses by Day 2, which was significantly different compared with vehicle or OSP. Body temperature drops over time were significantly greater with BXM than with vehicle or OSP. Significant reduction in virus titers was also demonstrated when BXM was administrated after symptom onset at Day 2 p.i. compared with vehicle and OSP, although body temperature changes largely overlapped between Day 2 and Day 4.
Conclusions: The results highlight the rapid antiviral action of BXM with post-exposure prophylaxis or therapeutic dosing in ferrets and offer support for further research on prevention of influenza virus infection and transmission.
Keywords: baloxavir marboxil; ferrets; influenza A virus; pharmacodynamics; pharmacokinetics.