tetano
Editor, Senior Moderator
Influenza Other Respir Viruses
. 2023 Mar 9;17(3):e13114.
doi: 10.1111/irv.13114. eCollection 2023 Mar.
SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus
Xiwen Wang[SUP] 1 2 [/SUP], Song Li[SUP] 1 [/SUP], Han Qu[SUP] 1 [/SUP], Liangyu Hao[SUP] 3 [/SUP], Ting Shao[SUP] 4 [/SUP], Kai Wang[SUP] 1 [/SUP], Zhiping Xia[SUP] 1 [/SUP], Zhiping Li[SUP] 1 [/SUP], Qianxue Li[SUP] 1 [/SUP]
Affiliations
Abstract
The surface-enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10[SUP]-6[/SUP] TCID[SUB]50[/SUB]/ml. The method showed excellent specificity with no cross-reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.
Keywords: H5N1 influenza virus; SERS; label‐free.
. 2023 Mar 9;17(3):e13114.
doi: 10.1111/irv.13114. eCollection 2023 Mar.
SERS-based immunomagnetic bead for rapid detection of H5N1 influenza virus
Xiwen Wang[SUP] 1 2 [/SUP], Song Li[SUP] 1 [/SUP], Han Qu[SUP] 1 [/SUP], Liangyu Hao[SUP] 3 [/SUP], Ting Shao[SUP] 4 [/SUP], Kai Wang[SUP] 1 [/SUP], Zhiping Xia[SUP] 1 [/SUP], Zhiping Li[SUP] 1 [/SUP], Qianxue Li[SUP] 1 [/SUP]
Affiliations
- PMID: 36909299
- PMCID: PMC9996428
- DOI: 10.1111/irv.13114
Abstract
The surface-enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10[SUP]-6[/SUP] TCID[SUB]50[/SUB]/ml. The method showed excellent specificity with no cross-reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.
Keywords: H5N1 influenza virus; SERS; label‐free.