tetano
Editor, Senior Moderator
Influenza Other Respir Viruses
. 2026 Jul;20(7):e70298.
doi: 10.1111/irv.70298.
Contribution of E190D and Q226H Mutations in HA to the Receptor-Binding Profile of D1.1 Genotype H5N1 Viruses
Yang Fang[SUP] 1 [/SUP], Lei Yang[SUP] 1 [/SUP], Shumei Zou[SUP] 1 [/SUP], Liqi Liu[SUP] 1 [/SUP], Wenfei Zhu[SUP] 1 [/SUP], Dayan Wang[SUP] 1 [/SUP]
Affiliations
A 2024 human HPAI H5N1 case in British Columbia showed mixed viral populations containing HA-190D (28%) and HA-226H (35%) variants in tracheal aspirate sequencing. In this study, solid-phase binding assay and molecular docking were used to evaluate the contribution of HA-E190D/Q226H mutations to the viral receptor profiles. Our results showed that HA-E190D marginally reduced sialic acid α2,3 receptors' affinity, while HA-Q226H impaired both sialic acid α2,3 and α2,6 receptors' binding. These results demonstrate that neither mutation strengthens viral binding to human-type receptors, indicating that such substitutions are unlikely to heighten the public health threat posed by the virus for now.
Keywords: E190D; HPAI H5N1; Q226H; public health threat; receptor profile.
. 2026 Jul;20(7):e70298.
doi: 10.1111/irv.70298.
Contribution of E190D and Q226H Mutations in HA to the Receptor-Binding Profile of D1.1 Genotype H5N1 Viruses
Yang Fang[SUP] 1 [/SUP], Lei Yang[SUP] 1 [/SUP], Shumei Zou[SUP] 1 [/SUP], Liqi Liu[SUP] 1 [/SUP], Wenfei Zhu[SUP] 1 [/SUP], Dayan Wang[SUP] 1 [/SUP]
Affiliations
- PMID: 42498495
- DOI: 10.1111/irv.70298
A 2024 human HPAI H5N1 case in British Columbia showed mixed viral populations containing HA-190D (28%) and HA-226H (35%) variants in tracheal aspirate sequencing. In this study, solid-phase binding assay and molecular docking were used to evaluate the contribution of HA-E190D/Q226H mutations to the viral receptor profiles. Our results showed that HA-E190D marginally reduced sialic acid α2,3 receptors' affinity, while HA-Q226H impaired both sialic acid α2,3 and α2,6 receptors' binding. These results demonstrate that neither mutation strengthens viral binding to human-type receptors, indicating that such substitutions are unlikely to heighten the public health threat posed by the virus for now.
Keywords: E190D; HPAI H5N1; Q226H; public health threat; receptor profile.