tetano
Editor, Senior Moderator
Sci Rep. 2017 Apr 13;7:46578. doi: 10.1038/srep46578.
[h=1]Influenza C in Lancaster, UK, in the winter of 2014-2015.[/h] Atkinson KV[SUP]1[/SUP], Bishop LA[SUP]1,[/SUP][SUP]2[/SUP], Rhodes G[SUP]3[/SUP], Salez N[SUP]4[/SUP], McEwan NR[SUP]5[/SUP], Hegarty MJ[SUP]5[/SUP], Robey J[SUP]6[/SUP], Harding N[SUP]6[/SUP], Wetherell S[SUP]6[/SUP], Lauder RM[SUP]1[/SUP], Pickup RW[SUP]1,[/SUP][SUP]3[/SUP], Wilkinson M[SUP]2[/SUP], Gatherer D[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza C is not included in the annual seasonal influenza vaccine, and has historically been regarded as a minor respiratory pathogen. However, recent work has highlighted its potential role as a cause of pneumonia in infants. We performed nasopharyngeal or nasal swabbing and/or serum sampling (n = 148) in Lancaster, UK, over the winter of 2014-2015. Using enzyme-linked immunosorbent assay (ELISA), we obtain seropositivity of 77%. By contrast, only 2 individuals, both asymptomatic adults, were influenza C-positive by polymerase chain reaction (PCR). Deep sequencing of nasopharyngeal samples produced partial sequences for 4 genome segments in one of these patients. Bayesian phylogenetic analysis demonstrated that the influenza C genome from this individual is evolutionarily distant to those sampled in recent years and represents a novel genome constellation, indicating that it may be a product of a decades-old reassortment event. Although we find no evidence that influenza C was a significant respiratory pathogen during the winter of 2014-2015 in Lancaster, we confirm previous observations of seropositivity in the majority of the population. (170 words).
PMID: 28406194 DOI: 10.1038/srep46578
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[h=1]Influenza C in Lancaster, UK, in the winter of 2014-2015.[/h] Atkinson KV[SUP]1[/SUP], Bishop LA[SUP]1,[/SUP][SUP]2[/SUP], Rhodes G[SUP]3[/SUP], Salez N[SUP]4[/SUP], McEwan NR[SUP]5[/SUP], Hegarty MJ[SUP]5[/SUP], Robey J[SUP]6[/SUP], Harding N[SUP]6[/SUP], Wetherell S[SUP]6[/SUP], Lauder RM[SUP]1[/SUP], Pickup RW[SUP]1,[/SUP][SUP]3[/SUP], Wilkinson M[SUP]2[/SUP], Gatherer D[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza C is not included in the annual seasonal influenza vaccine, and has historically been regarded as a minor respiratory pathogen. However, recent work has highlighted its potential role as a cause of pneumonia in infants. We performed nasopharyngeal or nasal swabbing and/or serum sampling (n = 148) in Lancaster, UK, over the winter of 2014-2015. Using enzyme-linked immunosorbent assay (ELISA), we obtain seropositivity of 77%. By contrast, only 2 individuals, both asymptomatic adults, were influenza C-positive by polymerase chain reaction (PCR). Deep sequencing of nasopharyngeal samples produced partial sequences for 4 genome segments in one of these patients. Bayesian phylogenetic analysis demonstrated that the influenza C genome from this individual is evolutionarily distant to those sampled in recent years and represents a novel genome constellation, indicating that it may be a product of a decades-old reassortment event. Although we find no evidence that influenza C was a significant respiratory pathogen during the winter of 2014-2015 in Lancaster, we confirm previous observations of seropositivity in the majority of the population. (170 words).
PMID: 28406194 DOI: 10.1038/srep46578
Free full text