tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2014 May 23. doi: 10.1111/irv.12247. [Epub ahead of print]
Influenza and other respiratory virus infections in outpatients with medically attended acute respiratory infection during the 2011-12 influenza season.
Zimmerman RK1, Rinaldo CR, Nowalk MP, Gk B, Thompson MG, Moehling KK, Bullotta A, Wisniewski S.
Author information
Abstract
BACKGROUND:
Respiratory tract infections are a major cause of outpatient visits, yet only a portion is tested to determine the etiologic organism. Multiplex reverse transcriptase polymerase chain reaction (MRT-PCR) assays for detection of multiple viruses are being used increasingly in clinical settings.
METHODS:
During January-April 2012, outpatients with acute respiratory illness (≤7 days) were tested for influenza using singleplex RT-PCR (SRT-PCR). A subset was assayed for 18 viruses using MRT-PCR to compare detection of influenza and examine the distribution of viruses and characteristics of patients using multinomial logistic regression.
RESULTS:
Among 662 participants (6 months-82 years), detection of influenza was similar between the MRT-PCR and SRT-PCR (κ = 0?83). No virus was identified in 267 (40.3%) samples. Commonly detected viruses were human rhinovirus (HRV, 15?4%), coronavirus (CoV, 10?4%), respiratory syncytial virus (RSV, 8?4%), human metapneumovirus (hMPV, 8?3%), and influenza (6%). Co-detections were infrequent (6?9%) and most commonly occurred among those <18 years old. In regression analyses, compared with non-viral illnesses, RSV and hMPV were significantly more frequent in children and less frequent in 18- to 49-year-olds than in those ≥50 years (P = 0?01), fever was more common in hMPV and influenza infections (P = 0?008), nasal congestion was more frequent in CoV, HRV, hMPV, influenza and RSV infections (P = 0?001), and body mass index was higher among those with influenza (P = 0?036).
CONCLUSIONS:
Using MRT-PCR, a viral etiology was found in three-fifths of patients with medically attended outpatient visits for acute respiratory illness during the influenza season; co-detected viruses were infrequent. Symptoms varied by viral etiology.
? 2014 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
KEYWORDS:
Co-detection, RT-PCR, influenza, viral infections
PMID:
24852890
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24852890
Influenza and other respiratory virus infections in outpatients with medically attended acute respiratory infection during the 2011-12 influenza season.
Zimmerman RK1, Rinaldo CR, Nowalk MP, Gk B, Thompson MG, Moehling KK, Bullotta A, Wisniewski S.
Author information
Abstract
BACKGROUND:
Respiratory tract infections are a major cause of outpatient visits, yet only a portion is tested to determine the etiologic organism. Multiplex reverse transcriptase polymerase chain reaction (MRT-PCR) assays for detection of multiple viruses are being used increasingly in clinical settings.
METHODS:
During January-April 2012, outpatients with acute respiratory illness (≤7 days) were tested for influenza using singleplex RT-PCR (SRT-PCR). A subset was assayed for 18 viruses using MRT-PCR to compare detection of influenza and examine the distribution of viruses and characteristics of patients using multinomial logistic regression.
RESULTS:
Among 662 participants (6 months-82 years), detection of influenza was similar between the MRT-PCR and SRT-PCR (κ = 0?83). No virus was identified in 267 (40.3%) samples. Commonly detected viruses were human rhinovirus (HRV, 15?4%), coronavirus (CoV, 10?4%), respiratory syncytial virus (RSV, 8?4%), human metapneumovirus (hMPV, 8?3%), and influenza (6%). Co-detections were infrequent (6?9%) and most commonly occurred among those <18 years old. In regression analyses, compared with non-viral illnesses, RSV and hMPV were significantly more frequent in children and less frequent in 18- to 49-year-olds than in those ≥50 years (P = 0?01), fever was more common in hMPV and influenza infections (P = 0?008), nasal congestion was more frequent in CoV, HRV, hMPV, influenza and RSV infections (P = 0?001), and body mass index was higher among those with influenza (P = 0?036).
CONCLUSIONS:
Using MRT-PCR, a viral etiology was found in three-fifths of patients with medically attended outpatient visits for acute respiratory illness during the influenza season; co-detected viruses were infrequent. Symptoms varied by viral etiology.
? 2014 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
KEYWORDS:
Co-detection, RT-PCR, influenza, viral infections
PMID:
24852890
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24852890