tetano
Editor, Senior Moderator
J Biol Chem. 2012 Mar 6. [Epub ahead of print]
Influenza A virus neuraminidase protein enhances cell survival through interaction with CEACAM6.
Gaur P, Ranjan P, Sharma S, Patel JR, Bowzard JB, Rahman SK, Kumari R, Gangappa S, Katz JM, Cox NJ, Lal RB, Sambhara S, Lal SK.
Source
ICGEB, India;
Abstract
The influenza virus neuraminidase (NA) protein primarily aids in the release of progeny virions from infected cells. Here, we demonstrate a novel role for NA in enhancing host cell survival by activating the Src/Akt signaling axis via an interaction with carcinoembryonic antigen-related cell adhesion molecule 6, CEACAM6/CD66c (C6). NA/C6 interaction lead to increased tyrosyl phosphorylation of Src, FAK, Akt, GSK3β and Bcl-2, which affects cell survival, proliferation, migration, differentiation and apoptosis. siRNA-mediated suppression of C6 resulted in a downregulation of activated Src, FAK, and Akt, increased apoptosis, and reduced expression of viral proteins and viral titers in influenza virus-infected A549 and NHBE cells. These findings indicate that influenza NA not only aids in the release of progeny virions, but also cell survival during viral replication.
PMID:
22396546
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22396546
Influenza A virus neuraminidase protein enhances cell survival through interaction with CEACAM6.
Gaur P, Ranjan P, Sharma S, Patel JR, Bowzard JB, Rahman SK, Kumari R, Gangappa S, Katz JM, Cox NJ, Lal RB, Sambhara S, Lal SK.
Source
ICGEB, India;
Abstract
The influenza virus neuraminidase (NA) protein primarily aids in the release of progeny virions from infected cells. Here, we demonstrate a novel role for NA in enhancing host cell survival by activating the Src/Akt signaling axis via an interaction with carcinoembryonic antigen-related cell adhesion molecule 6, CEACAM6/CD66c (C6). NA/C6 interaction lead to increased tyrosyl phosphorylation of Src, FAK, Akt, GSK3β and Bcl-2, which affects cell survival, proliferation, migration, differentiation and apoptosis. siRNA-mediated suppression of C6 resulted in a downregulation of activated Src, FAK, and Akt, increased apoptosis, and reduced expression of viral proteins and viral titers in influenza virus-infected A549 and NHBE cells. These findings indicate that influenza NA not only aids in the release of progeny virions, but also cell survival during viral replication.
PMID:
22396546
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22396546