tetano
Editor, Senior Moderator
J Virol. 2015 Dec 4. pii: JVI.02246-15. [Epub ahead of print]
[h=1]Influenza A virus-induced expression of a GalNAc transferase, GALNT3, via miRNAs is required for enhanced viral replication.[/h] Nakamura S[SUP]1[/SUP], Horie M[SUP]2[/SUP], Daidoji T[SUP]3[/SUP], Honda T[SUP]2[/SUP], Yasugi M[SUP]4[/SUP], Kuno A[SUP]5[/SUP], Komori T[SUP]6[/SUP], Okuzaki D[SUP]7[/SUP], Narimatsu H[SUP]5[/SUP], Nakaya T[SUP]4[/SUP], Tomonaga K[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus (IAV) affects the upper and lower respiratory tracts and rapidly induces the expression of mucins, which are common O-glycosylated proteins, on the epithelial surfaces of the respiratory tract. Although mucin production is associated with the inhibition of virus transmission as well as characteristic clinical symptoms, little is known regarding how mucins are produced on the surfaces of respiratory epithelial cells and how they affect IAV replication. In this study we found that two miRNAs, miR-17-3p and miR-221, which target GalNAc transferase 3 (GALNT3) mRNA, are rapidly downregulated in human alveolar basal epithelial cells during the early stage of IAV expression. We demonstrated that the expression of GALNT3 mRNA is upregulated in an IAV replication-dependent fashion and leads to mucin production in bronchial epithelial cells. A lectin microarray analysis revealed that the stable expression of GALNT3 by human alveolar basal epithelial cells induces mucin-type O-glycosylation modifications similar to those present in IAV-infected cells, suggesting that GALNT3 promotes mucin-type O-linked glycosylation in IAV-infected cells. Notably, analyses using siRNAs and miRNA mimics showed that GALNT3 knockdown significantly reduces IAV replication. Furthermore, IAV replication was markedly decreased in embryonic fibroblast cells obtained from galnt3-knockout mice. Interestingly, IAV-infected galnt3-knockout mice exhibited high mortality and severe pathological alterations in the lungs compared to wild type mice. Our results demonstrate not only the molecular mechanism underlying rapid mucin production during IAV infection, but also the contribution of O-linked glycosylation to the replication and propagation of IAV in lung cells.
[h=4]IMPORTANCE:[/h] Viral infections that affect the upper or lower respiratory tracts, such as Influenza A virus (IAV), rapidly induce mucin production on the epithelial surfaces of respiratory cells. However, the details of how mucin-type O-linked glycosylation is initiated by IAV infection and how mucin production affects viral replication have not yet been elucidated. In this study, we show that two miRNAs that target the UDP-GalNAc transferase GALNT3 are markedly decreased during the early stage of IAV infection, resulting in the upregulation of GALNT3 mRNA. We also demonstrate that the expression of GALNT3 initiates mucin production and affects IAV replication in infected cells. This is the first report demonstrating the mechanism underlying the miRNA-mediated initiation of mucin-type O-glycosylation in IAV-infected cells and its role in viral replication. Our results have broad implications for understanding IAV replication and suggest a strategy for the development of novel anti-influenza approaches.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26637460 [PubMed - as supplied by publisher]
[h=1]Influenza A virus-induced expression of a GalNAc transferase, GALNT3, via miRNAs is required for enhanced viral replication.[/h] Nakamura S[SUP]1[/SUP], Horie M[SUP]2[/SUP], Daidoji T[SUP]3[/SUP], Honda T[SUP]2[/SUP], Yasugi M[SUP]4[/SUP], Kuno A[SUP]5[/SUP], Komori T[SUP]6[/SUP], Okuzaki D[SUP]7[/SUP], Narimatsu H[SUP]5[/SUP], Nakaya T[SUP]4[/SUP], Tomonaga K[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus (IAV) affects the upper and lower respiratory tracts and rapidly induces the expression of mucins, which are common O-glycosylated proteins, on the epithelial surfaces of the respiratory tract. Although mucin production is associated with the inhibition of virus transmission as well as characteristic clinical symptoms, little is known regarding how mucins are produced on the surfaces of respiratory epithelial cells and how they affect IAV replication. In this study we found that two miRNAs, miR-17-3p and miR-221, which target GalNAc transferase 3 (GALNT3) mRNA, are rapidly downregulated in human alveolar basal epithelial cells during the early stage of IAV expression. We demonstrated that the expression of GALNT3 mRNA is upregulated in an IAV replication-dependent fashion and leads to mucin production in bronchial epithelial cells. A lectin microarray analysis revealed that the stable expression of GALNT3 by human alveolar basal epithelial cells induces mucin-type O-glycosylation modifications similar to those present in IAV-infected cells, suggesting that GALNT3 promotes mucin-type O-linked glycosylation in IAV-infected cells. Notably, analyses using siRNAs and miRNA mimics showed that GALNT3 knockdown significantly reduces IAV replication. Furthermore, IAV replication was markedly decreased in embryonic fibroblast cells obtained from galnt3-knockout mice. Interestingly, IAV-infected galnt3-knockout mice exhibited high mortality and severe pathological alterations in the lungs compared to wild type mice. Our results demonstrate not only the molecular mechanism underlying rapid mucin production during IAV infection, but also the contribution of O-linked glycosylation to the replication and propagation of IAV in lung cells.
[h=4]IMPORTANCE:[/h] Viral infections that affect the upper or lower respiratory tracts, such as Influenza A virus (IAV), rapidly induce mucin production on the epithelial surfaces of respiratory cells. However, the details of how mucin-type O-linked glycosylation is initiated by IAV infection and how mucin production affects viral replication have not yet been elucidated. In this study, we show that two miRNAs that target the UDP-GalNAc transferase GALNT3 are markedly decreased during the early stage of IAV infection, resulting in the upregulation of GALNT3 mRNA. We also demonstrate that the expression of GALNT3 initiates mucin production and affects IAV replication in infected cells. This is the first report demonstrating the mechanism underlying the miRNA-mediated initiation of mucin-type O-glycosylation in IAV-infected cells and its role in viral replication. Our results have broad implications for understanding IAV replication and suggest a strategy for the development of novel anti-influenza approaches.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26637460 [PubMed - as supplied by publisher]