tetano
Editor, Senior Moderator
Infection
. 2020 Aug 12.
doi: 10.1007/s15010-020-01497-2. Online ahead of print.
Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
Marianna Theresia Traugott[SUP] 1 [/SUP], Wolfgang Hoepler[SUP] 1 [/SUP], Tamara Seitz[SUP] 1 [/SUP], Sebastian Baumgartner[SUP] 1 [/SUP], Mario Karolyi[SUP] 1 [/SUP], Erich Pawelka[SUP] 1 [/SUP], Emanuela Friese[SUP] 1 [/SUP], Stephanie Neuhold[SUP] 1 [/SUP], Hasan Kelani[SUP] 1 [/SUP], Florian Thalhammer[SUP] 2 [/SUP], Alexander Zoufaly[SUP] 1 [/SUP], Hermann Laferl[SUP] 1 [/SUP], Judith Helene Aberle[SUP] 3 [/SUP], Christoph Wenisch[SUP] 1 [/SUP], Elisabeth Puchhammer-St?ckl[SUP] 3 [/SUP], Karin Stiasny[SUP] 3 [/SUP], Stephan Walter Aberle[SUP] 3 [/SUP], Lukas Weseslindtner[SUP] 4 [/SUP]
Affiliations
Abstract
We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight that commercial ELISAs and even rapid tests might significantly aid the diagnosis of COVID-19, particularly, if a combination of serological assays is used with a specific clinical question, in severely ill patients after seroconversion and when comprehensive serological methods are used for confirmation.
Keywords: Antibodies; Coronavirus; Immunoassay; PCR negative; Rapid test; SARS.
. 2020 Aug 12.
doi: 10.1007/s15010-020-01497-2. Online ahead of print.
Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
Marianna Theresia Traugott[SUP] 1 [/SUP], Wolfgang Hoepler[SUP] 1 [/SUP], Tamara Seitz[SUP] 1 [/SUP], Sebastian Baumgartner[SUP] 1 [/SUP], Mario Karolyi[SUP] 1 [/SUP], Erich Pawelka[SUP] 1 [/SUP], Emanuela Friese[SUP] 1 [/SUP], Stephanie Neuhold[SUP] 1 [/SUP], Hasan Kelani[SUP] 1 [/SUP], Florian Thalhammer[SUP] 2 [/SUP], Alexander Zoufaly[SUP] 1 [/SUP], Hermann Laferl[SUP] 1 [/SUP], Judith Helene Aberle[SUP] 3 [/SUP], Christoph Wenisch[SUP] 1 [/SUP], Elisabeth Puchhammer-St?ckl[SUP] 3 [/SUP], Karin Stiasny[SUP] 3 [/SUP], Stephan Walter Aberle[SUP] 3 [/SUP], Lukas Weseslindtner[SUP] 4 [/SUP]
Affiliations
- PMID: 32785885
- DOI: 10.1007/s15010-020-01497-2
Abstract
We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight that commercial ELISAs and even rapid tests might significantly aid the diagnosis of COVID-19, particularly, if a combination of serological assays is used with a specific clinical question, in severely ill patients after seroconversion and when comprehensive serological methods are used for confirmation.
Keywords: Antibodies; Coronavirus; Immunoassay; PCR negative; Rapid test; SARS.