tetano
Editor, Senior Moderator
Infect Genet Evol
. 2021 Jun 18;104972.
doi: 10.1016/j.meegid.2021.104972. Online ahead of print.
Molecular and biological characterization of influenza A viruses isolated from human fecal samples
Hebah A Al Khatib[SUP] 1 [/SUP], Peter V Coyle[SUP] 2 [/SUP], Muna A Al Maslamani[SUP] 3 [/SUP], Asmaa A Al Thani[SUP] 4 [/SUP], Sameer A Pathan[SUP] 5 [/SUP], Hadi M Yassine[SUP] 6 [/SUP]
Affiliations
Abstract
Human influenza viruses are occasionally detected in the stools of influenza patients.
Objectives: Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission.
Methods: Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated.
Results: Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors.
Conclusion: The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission.
Keywords: Influenza virus; Intestinal replication; Sialic acid receptors; Transmission; Virus diversity.
. 2021 Jun 18;104972.
doi: 10.1016/j.meegid.2021.104972. Online ahead of print.
Molecular and biological characterization of influenza A viruses isolated from human fecal samples
Hebah A Al Khatib[SUP] 1 [/SUP], Peter V Coyle[SUP] 2 [/SUP], Muna A Al Maslamani[SUP] 3 [/SUP], Asmaa A Al Thani[SUP] 4 [/SUP], Sameer A Pathan[SUP] 5 [/SUP], Hadi M Yassine[SUP] 6 [/SUP]
Affiliations
- PMID: 34153546
- DOI: 10.1016/j.meegid.2021.104972
Abstract
Human influenza viruses are occasionally detected in the stools of influenza patients.
Objectives: Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission.
Methods: Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated.
Results: Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors.
Conclusion: The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission.
Keywords: Influenza virus; Intestinal replication; Sialic acid receptors; Transmission; Virus diversity.