tetano
Editor, Senior Moderator
Infect Genet Evol
. 2022 Dec 27;105399.
doi: 10.1016/j.meegid.2022.105399. Online ahead of print.
Bats-associated beta-coronavirus detection and characterization: First report from Pakistan
Sidra Rahman[SUP] 1 [/SUP], Sana Ullah[SUP] 2 [/SUP], Zabta Khan Shinwari[SUP] 3 [/SUP], Muhammad Ali[SUP] 4 [/SUP]
Affiliations
Abstract
Bats remains as reservoirs for highly contagious and pathogenic viral families including the Coronaviridae, Filoviridae, Paramyxoviruses, and Rhabdoviridae. Spill over of viral species (SARS-CoV, MERS-CoV & SARS-CoV2) from bats (as a possible potential reservoirs) have recently caused worst outbreaks. Early detection of viral species of pandemic potential in bats is of great importance. We detected beta coronaviruses in the studied bats population (positive samples from Rousettus leschenaultia) and performed the evolutionary analysis, amino acid sequence alignment, and analysed the 3-Dimentional protein structure. We detected the coronaviruses for the first time in bats from Pakistan. Our analysis based on RdRp partial gene sequencing suggest that the studied viral strains are closely related to MERS-CoV like viruses as they exhibit close structure similarities (with few substitutions) and also observed a substitution in highly conserved SDD in the palm subdomain of motif C to ADD, when compared with earlier reported viral strains. It could be concluded from our study that coronaviruses are circulating among the bat's population in Pakistan. Based on the current findings, we suggest large scale screening procedures of bat virome across the country to detect potential pathogenic viral species.
Keywords: Bats-borne viruses; Coronaviruses; MERS; Molecular detection; Zoonotic diseases.
. 2022 Dec 27;105399.
doi: 10.1016/j.meegid.2022.105399. Online ahead of print.
Bats-associated beta-coronavirus detection and characterization: First report from Pakistan
Sidra Rahman[SUP] 1 [/SUP], Sana Ullah[SUP] 2 [/SUP], Zabta Khan Shinwari[SUP] 3 [/SUP], Muhammad Ali[SUP] 4 [/SUP]
Affiliations
- PMID: 36584905
- PMCID: PMC9793958
- DOI: 10.1016/j.meegid.2022.105399
Abstract
Bats remains as reservoirs for highly contagious and pathogenic viral families including the Coronaviridae, Filoviridae, Paramyxoviruses, and Rhabdoviridae. Spill over of viral species (SARS-CoV, MERS-CoV & SARS-CoV2) from bats (as a possible potential reservoirs) have recently caused worst outbreaks. Early detection of viral species of pandemic potential in bats is of great importance. We detected beta coronaviruses in the studied bats population (positive samples from Rousettus leschenaultia) and performed the evolutionary analysis, amino acid sequence alignment, and analysed the 3-Dimentional protein structure. We detected the coronaviruses for the first time in bats from Pakistan. Our analysis based on RdRp partial gene sequencing suggest that the studied viral strains are closely related to MERS-CoV like viruses as they exhibit close structure similarities (with few substitutions) and also observed a substitution in highly conserved SDD in the palm subdomain of motif C to ADD, when compared with earlier reported viral strains. It could be concluded from our study that coronaviruses are circulating among the bat's population in Pakistan. Based on the current findings, we suggest large scale screening procedures of bat virome across the country to detect potential pathogenic viral species.
Keywords: Bats-borne viruses; Coronaviruses; MERS; Molecular detection; Zoonotic diseases.