tetano
Editor, Senior Moderator
Infect Dis Ther
. 2021 Jan 8.
doi: 10.1007/s40121-020-00381-8. Online ahead of print.
Prevalence and Clinical Significance of Persistent Viral Shedding in Hospitalized Adult Patients with SARS-CoV-2 Infection: A Prospective Observational Study
Antonio Vena[SUP] 1 [/SUP], Lucia Taramasso[SUP] 1 [/SUP], Antonio Di Biagio[SUP] 1 2 [/SUP], Malgorzata Mikulska[SUP] 1 2 [/SUP], Chiara Dentone[SUP] 1 [/SUP], Andrea De Maria[SUP] 1 2 [/SUP], Laura Magnasco[SUP] 1 [/SUP], Laura Ambra Nicolini[SUP] 1 [/SUP], Bianca Bruzzone[SUP] 3 [/SUP], Giancarlo Icardi[SUP] 2 3 [/SUP], Andrea Orsi[SUP] 2 3 [/SUP], Paolo Pelosi[SUP] 4 5 6 [/SUP], Lorenzo Ball[SUP] 4 5 6 [/SUP], Denise Battaglini[SUP] 5 6 [/SUP], Iole Brunetti[SUP] 5 6 [/SUP], Maurizio Loconte[SUP] 5 6 [/SUP], Nicol? A Patroniti[SUP] 4 5 6 [/SUP], Chiara Robba[SUP] 5 6 [/SUP], Martina Bavastro[SUP] 1 [/SUP], Matteo Cerchiaro[SUP] 1 [/SUP], Daniele Roberto Giacobbe[SUP] 1 [/SUP], Irene Schiavetti[SUP] 7 [/SUP], Marco Berruti[SUP] 1 2 [/SUP], Matteo Bassetti[SUP] 8 9 [/SUP], GECOVID study group
Collaborators, Affiliations
Abstract
Background: The goal of this study was to investigate the prevalence and factors associated with persistent viral shedding (PVS) in hospitalized patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Methods: This was a prospective observational study including all consecutive adults hospitalized with SARS-CoV-2 infection. When the first nasopharyngeal swab was positive for SARS-CoV-2 RNA (day 0), additional samples were obtained on days + 3, + 5, + 7 and then once every 7 days until virus detection was negative. PVS was defined as the duration of shedding of at least 21 days after diagnosis. The primary endpoint of this study was the prevalence of PVS.
Results: Data were obtained regarding 121 consecutive hospitalized patients with SARS-CoV-2 infection (median age 66 years, male sex 65.3%). Overall, the prevalence of PVS was 38% (46/121 patients). According to univariate analysis, factors associated with PVS were immunosuppression (6.7% vs 21.7%, p = 0.02), increased interleukin-6 (IL-6) levels (≥ 35 ng/ml) at the time of diagnosis (43.4% vs 67.3%, p = 0.02), time from onset of symptoms to diagnosis (median days 7.0 vs 3.5, p = 0.001), intensive care unit admission (22.7% vs 43.5%, p = 0.02), and need for invasive mechanical ventilation (20.0% vs 41.3%, p = 0.01). The multivariate analysis indicated that immunosuppression, increased IL-6 levels at the time of diagnosis, time from onset of symptoms to diagnosis, and need for mechanical ventilation were independent factors associated with PVS.
Conclusions: PVS was detected in up to 38% of hospitalized patients with SARS-CoV-2 infection and was strongly associated with immunosuppression, increased IL-6 levels, and the need for mechanical ventilation.
Keywords: COVID-19; IL-6; Outcome; SARS-CoV-2; Viral shedding.
. 2021 Jan 8.
doi: 10.1007/s40121-020-00381-8. Online ahead of print.
Prevalence and Clinical Significance of Persistent Viral Shedding in Hospitalized Adult Patients with SARS-CoV-2 Infection: A Prospective Observational Study
Antonio Vena[SUP] 1 [/SUP], Lucia Taramasso[SUP] 1 [/SUP], Antonio Di Biagio[SUP] 1 2 [/SUP], Malgorzata Mikulska[SUP] 1 2 [/SUP], Chiara Dentone[SUP] 1 [/SUP], Andrea De Maria[SUP] 1 2 [/SUP], Laura Magnasco[SUP] 1 [/SUP], Laura Ambra Nicolini[SUP] 1 [/SUP], Bianca Bruzzone[SUP] 3 [/SUP], Giancarlo Icardi[SUP] 2 3 [/SUP], Andrea Orsi[SUP] 2 3 [/SUP], Paolo Pelosi[SUP] 4 5 6 [/SUP], Lorenzo Ball[SUP] 4 5 6 [/SUP], Denise Battaglini[SUP] 5 6 [/SUP], Iole Brunetti[SUP] 5 6 [/SUP], Maurizio Loconte[SUP] 5 6 [/SUP], Nicol? A Patroniti[SUP] 4 5 6 [/SUP], Chiara Robba[SUP] 5 6 [/SUP], Martina Bavastro[SUP] 1 [/SUP], Matteo Cerchiaro[SUP] 1 [/SUP], Daniele Roberto Giacobbe[SUP] 1 [/SUP], Irene Schiavetti[SUP] 7 [/SUP], Marco Berruti[SUP] 1 2 [/SUP], Matteo Bassetti[SUP] 8 9 [/SUP], GECOVID study group
Collaborators, Affiliations
- PMID: 33417230
- DOI: 10.1007/s40121-020-00381-8
Abstract
Background: The goal of this study was to investigate the prevalence and factors associated with persistent viral shedding (PVS) in hospitalized patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Methods: This was a prospective observational study including all consecutive adults hospitalized with SARS-CoV-2 infection. When the first nasopharyngeal swab was positive for SARS-CoV-2 RNA (day 0), additional samples were obtained on days + 3, + 5, + 7 and then once every 7 days until virus detection was negative. PVS was defined as the duration of shedding of at least 21 days after diagnosis. The primary endpoint of this study was the prevalence of PVS.
Results: Data were obtained regarding 121 consecutive hospitalized patients with SARS-CoV-2 infection (median age 66 years, male sex 65.3%). Overall, the prevalence of PVS was 38% (46/121 patients). According to univariate analysis, factors associated with PVS were immunosuppression (6.7% vs 21.7%, p = 0.02), increased interleukin-6 (IL-6) levels (≥ 35 ng/ml) at the time of diagnosis (43.4% vs 67.3%, p = 0.02), time from onset of symptoms to diagnosis (median days 7.0 vs 3.5, p = 0.001), intensive care unit admission (22.7% vs 43.5%, p = 0.02), and need for invasive mechanical ventilation (20.0% vs 41.3%, p = 0.01). The multivariate analysis indicated that immunosuppression, increased IL-6 levels at the time of diagnosis, time from onset of symptoms to diagnosis, and need for mechanical ventilation were independent factors associated with PVS.
Conclusions: PVS was detected in up to 38% of hospitalized patients with SARS-CoV-2 infection and was strongly associated with immunosuppression, increased IL-6 levels, and the need for mechanical ventilation.
Keywords: COVID-19; IL-6; Outcome; SARS-CoV-2; Viral shedding.