tetano
Editor, Senior Moderator
Infect Dis Rep
. 2021 Dec 14;13(4):1061-1077.
doi: 10.3390/idr13040097.
A Sensitive, Portable Microfluidic Device for SARS-CoV-2 Detection from Self-Collected Saliva
Jianing Yang[SUP] 1 [/SUP], Mark Kidd[SUP] 2 [/SUP], Alan R Nordquist[SUP] 1 [/SUP], Stanley D Smith[SUP] 1 [/SUP], Cedric Hurth[SUP] 1 [/SUP], Irvin M Modlin[SUP] 2 [/SUP], Frederic Zenhausern[SUP] 1 [/SUP]
Affiliations
Abstract
Since the outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in December 2019, the spread of SARS-CoV2 infection has been escalating rapidly around the world. In order to provide more timely access to medical intervention, including diagnostic tests and medical treatment, the FDA authorized multiple test protocols for diagnostic tests from nasopharyngeal swab, saliva, urine, bronchoalveolar lavage and fecal samples. The traditional diagnostic tests for this novel coronavirus 2019 require standard processes of viral RNA isolation, reverse transcription of RNA to cDNA, then real-time quantitative PCR with the RNA templates extracted from the patient samples. Recently, many reports have demonstrated a direct detection of SARS-Co-V2 genomic material from saliva samples without any RNA isolation step. To make the rapid detection of SARS-Co-V2 infection more accessible, a point-of-care type device was developed for SARS-CoV-2 detection. Herein, we report a portable microfluidic-based integrated detection-analysis system for SARS-CoV-2 nucleic acids detection directly from saliva samples. The saliva cartridge is self-contained and capable of microfluidic evaluation of saliva, from heating, mixing with the primers to multiplex real-time quantitative polymerase chain reaction, detecting SARS-CoV-2 with different primer sets and internal control. The approach has a detection sensitivity of 1000 copies/mL of SARS-CoV-2 RNA or virus, with consistency and automation, from saliva sample-in to result-out.
Keywords: SARS-CoV-2; cartridge; detection; integration; microfluidics; saliva; sensitivity.
. 2021 Dec 14;13(4):1061-1077.
doi: 10.3390/idr13040097.
A Sensitive, Portable Microfluidic Device for SARS-CoV-2 Detection from Self-Collected Saliva
Jianing Yang[SUP] 1 [/SUP], Mark Kidd[SUP] 2 [/SUP], Alan R Nordquist[SUP] 1 [/SUP], Stanley D Smith[SUP] 1 [/SUP], Cedric Hurth[SUP] 1 [/SUP], Irvin M Modlin[SUP] 2 [/SUP], Frederic Zenhausern[SUP] 1 [/SUP]
Affiliations
- PMID: 34940407
- DOI: 10.3390/idr13040097
Abstract
Since the outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in December 2019, the spread of SARS-CoV2 infection has been escalating rapidly around the world. In order to provide more timely access to medical intervention, including diagnostic tests and medical treatment, the FDA authorized multiple test protocols for diagnostic tests from nasopharyngeal swab, saliva, urine, bronchoalveolar lavage and fecal samples. The traditional diagnostic tests for this novel coronavirus 2019 require standard processes of viral RNA isolation, reverse transcription of RNA to cDNA, then real-time quantitative PCR with the RNA templates extracted from the patient samples. Recently, many reports have demonstrated a direct detection of SARS-Co-V2 genomic material from saliva samples without any RNA isolation step. To make the rapid detection of SARS-Co-V2 infection more accessible, a point-of-care type device was developed for SARS-CoV-2 detection. Herein, we report a portable microfluidic-based integrated detection-analysis system for SARS-CoV-2 nucleic acids detection directly from saliva samples. The saliva cartridge is self-contained and capable of microfluidic evaluation of saliva, from heating, mixing with the primers to multiplex real-time quantitative polymerase chain reaction, detecting SARS-CoV-2 with different primer sets and internal control. The approach has a detection sensitivity of 1000 copies/mL of SARS-CoV-2 RNA or virus, with consistency and automation, from saliva sample-in to result-out.
Keywords: SARS-CoV-2; cartridge; detection; integration; microfluidics; saliva; sensitivity.