tetano
Editor, Senior Moderator
J Gen Virol. 2016 Dec 15. doi: 10.1099/jgv.0.000683. [Epub ahead of print]
[h=1]Induction of protective immunity against influenza A/Jiangxi-Donghu/346/2013 (H10N8) in mice.[/h] Kuah LF[SUP]1[/SUP], Tang LH[SUP]2[/SUP], Sutton T[SUP]3[/SUP], Lim JH[SUP]4[/SUP], Sin WL[SUP]5[/SUP], Lamirande E[SUP]6[/SUP], Subbarao K[SUP]7[/SUP], Lau YF[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Human infections with A/Jiangxi-Donghu/346/2013 (H10N8) virus have raised concerns about its pandemic potential. In order to develop a vaccine against this virus, the immunogenicity of its hemagglutinin (HA) protein was evaluated in mice. Using both whole-virion and recombinant subunit protein vaccines, we showed that two doses of either vaccine elicited neutralizing antibody responses. The protective efficacy of the vaccine-induced responses was assessed using a reverse-genetics- derived H10 reassortant virus on the A/Puerto Rico/8/34 (H1N1) backbone. The reassortant virus replicated efficiently in the respiratory tract of unvaccinated mice while vaccinated mice were completely protected from challenge, with no detectable viral load in the lower respiratory tract. Finally, the serum neutralizing antibody responses elicited by the H10 vaccines also exhibited cross-neutralizing activity against three heterologous wild-type H10 viruses. Collectively, these findings demonstrate that different vaccine platforms presenting the H10 HA protein induce protective immunity.
PMID: 27983474 DOI: 10.1099/jgv.0.000683
[PubMed - as supplied by publisher]
[h=1]Induction of protective immunity against influenza A/Jiangxi-Donghu/346/2013 (H10N8) in mice.[/h] Kuah LF[SUP]1[/SUP], Tang LH[SUP]2[/SUP], Sutton T[SUP]3[/SUP], Lim JH[SUP]4[/SUP], Sin WL[SUP]5[/SUP], Lamirande E[SUP]6[/SUP], Subbarao K[SUP]7[/SUP], Lau YF[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Human infections with A/Jiangxi-Donghu/346/2013 (H10N8) virus have raised concerns about its pandemic potential. In order to develop a vaccine against this virus, the immunogenicity of its hemagglutinin (HA) protein was evaluated in mice. Using both whole-virion and recombinant subunit protein vaccines, we showed that two doses of either vaccine elicited neutralizing antibody responses. The protective efficacy of the vaccine-induced responses was assessed using a reverse-genetics- derived H10 reassortant virus on the A/Puerto Rico/8/34 (H1N1) backbone. The reassortant virus replicated efficiently in the respiratory tract of unvaccinated mice while vaccinated mice were completely protected from challenge, with no detectable viral load in the lower respiratory tract. Finally, the serum neutralizing antibody responses elicited by the H10 vaccines also exhibited cross-neutralizing activity against three heterologous wild-type H10 viruses. Collectively, these findings demonstrate that different vaccine platforms presenting the H10 HA protein induce protective immunity.
PMID: 27983474 DOI: 10.1099/jgv.0.000683
[PubMed - as supplied by publisher]