tetano
Editor, Senior Moderator
Sci Rep. 2018 Dec 20;8(1):18007. doi: 10.1038/s41598-018-36703-7.
[h=1]Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine.[/h] Skibinski DAG[SUP]1,[/SUP][SUP]2[/SUP], Jones LA[SUP]1[/SUP], Zhu YO[SUP]3[/SUP], Xue LW[SUP]1[/SUP], Au B[SUP]1[/SUP], Lee B[SUP]4[/SUP], Naim ANM[SUP]3[/SUP], Lee A[SUP]1[/SUP], Kaliaperumal N[SUP]1[/SUP], Low JGH[SUP]5,[/SUP][SUP]6[/SUP], Lee LS[SUP]7,[/SUP][SUP]8[/SUP], Poidinger M[SUP]4[/SUP], Saudan P[SUP]9[/SUP], Bachmann M[SUP]10[/SUP], Ooi EE[SUP]11[/SUP], Hanson BJ[SUP]12[/SUP], Novotny-Diermayr V[SUP]13[/SUP], Matter A[SUP]13[/SUP], Fairhurst AM[SUP]4[/SUP], Hibberd ML[SUP]3[/SUP], Connolly JE[SUP]14,[/SUP][SUP]15,[/SUP][SUP]16[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Cell mediated immunity plays a vital role in defense against influenza infection in humans. Less is known about the role of vaccine-induced cell mediated immunity and the cytokine responses elicited. We measured CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell reactivity in human subjects following vaccination with licensed trivalent influenza vaccine and a novel virus-like particle based vaccine. We detected influenza-specific CD4[SUP]+[/SUP] T-cell responses following vaccination with the licensed trivalent influenza vaccine and found that these correlated with antibody measurements. Administration of the novel virus-like particle based vaccine elicited influenza-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell responses and the induction of the cytokines IFN-γ, IL-17A, IL17F, IL-5, IL-13, IL-9, IL-10 and IL-21. Pre-existing cytokine responses influenced the profile of the cytokine response elicited by vaccination. In a subset of individuals the VLP vaccine changed pre-vaccination production of type 2 cytokines such as IL-5 and IL-13 to a post-vaccination type 1 cytokine signature characterized by IFN-γ. A transcriptional signature to vaccination was found to correlate with antibody titer, IFN-γ production by T-cells and expression of a putative RNA helicase, DDX17, on the surface of immune cells.
PMID: 30573748 PMCID: PMC6301966 DOI: 10.1038/s41598-018-36703-7
Free PMC Article
[h=1]Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine.[/h] Skibinski DAG[SUP]1,[/SUP][SUP]2[/SUP], Jones LA[SUP]1[/SUP], Zhu YO[SUP]3[/SUP], Xue LW[SUP]1[/SUP], Au B[SUP]1[/SUP], Lee B[SUP]4[/SUP], Naim ANM[SUP]3[/SUP], Lee A[SUP]1[/SUP], Kaliaperumal N[SUP]1[/SUP], Low JGH[SUP]5,[/SUP][SUP]6[/SUP], Lee LS[SUP]7,[/SUP][SUP]8[/SUP], Poidinger M[SUP]4[/SUP], Saudan P[SUP]9[/SUP], Bachmann M[SUP]10[/SUP], Ooi EE[SUP]11[/SUP], Hanson BJ[SUP]12[/SUP], Novotny-Diermayr V[SUP]13[/SUP], Matter A[SUP]13[/SUP], Fairhurst AM[SUP]4[/SUP], Hibberd ML[SUP]3[/SUP], Connolly JE[SUP]14,[/SUP][SUP]15,[/SUP][SUP]16[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Cell mediated immunity plays a vital role in defense against influenza infection in humans. Less is known about the role of vaccine-induced cell mediated immunity and the cytokine responses elicited. We measured CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell reactivity in human subjects following vaccination with licensed trivalent influenza vaccine and a novel virus-like particle based vaccine. We detected influenza-specific CD4[SUP]+[/SUP] T-cell responses following vaccination with the licensed trivalent influenza vaccine and found that these correlated with antibody measurements. Administration of the novel virus-like particle based vaccine elicited influenza-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T-cell responses and the induction of the cytokines IFN-γ, IL-17A, IL17F, IL-5, IL-13, IL-9, IL-10 and IL-21. Pre-existing cytokine responses influenced the profile of the cytokine response elicited by vaccination. In a subset of individuals the VLP vaccine changed pre-vaccination production of type 2 cytokines such as IL-5 and IL-13 to a post-vaccination type 1 cytokine signature characterized by IFN-γ. A transcriptional signature to vaccination was found to correlate with antibody titer, IFN-γ production by T-cells and expression of a putative RNA helicase, DDX17, on the surface of immune cells.
PMID: 30573748 PMCID: PMC6301966 DOI: 10.1038/s41598-018-36703-7
Free PMC Article